In order to identify the species of parasites found in the fecal samples of C57BL/6 mice introduced from a unit, the mor-phology of parasites was observed and the phylogenetic analysis was carried out. The fecal smears of C57BL/6 mice were stained with Swiss stain and observed under microscope. The total DNA of fecal samples was extracted, the 16S rRNA of Tritrichomonas murism was amplified by PCR and sequenced, Mega11 was used for phylogenetic analysis. Microscopical observation showed that the parasite conformed to the morphological characteristics of Tritrichomonas minuta, and sequencing showed that the gene sequence of Tritri-chomonas minuta was highly homologous with that of Tritrichomonas murism.
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