Differential expression profiles of miRNA in exosomes derived from hepatocytes of muscovy ducks immunized by live vaccine MDRV infection
To explore the effect of live vaccine muscovy duck reovirus(MDRV) on microRNA(miRNA) in hepatocytes-derived exosomes of muscovy duck, forty 1-day-old muscovy ducklings were randomly divided into experimental group and control group, with 20 ducks in each group. The experimental group was intramuscularly injected with 1 dose of MDRV live vaccine, and the control group was in-jected with the same amount of sterilized normal saline. At 48 hours post immunization, the livers were collected to prepare primary liver cells and they were culture for 2 days, the exosomes from supernatant were extracted by exosome extraction kit, and were sent to a biological company for miRNA sequencing, annotation, differential expression analysis, target gene prediction, GO and KEGG en-richment analysis of the biological functions involved in the target genes were performed. The results showed that a total of 39 signifi-cantly different expression miRNAs were screened in the live vaccine MDRV immunization group compared with the control group, which targeted 369 mRNA. The GO functional annotations mainly focused on single-organism processes, stimulus responses, multicel-lular organism processes, localization, organelles, membranes, extracellular regions, and binding molecular functions. The result of KEGG pathway enrichment analysis mainly involved in the metabolism of amino sugars and nucleotide sugars, glycolysis/gluconeogen-esis, lysosomes, phosphatidylinositol signaling system, inositol phosphate metabolism, apoptosis, and cell autophagocytosis in the liver. These results revealed that muscovy ducklings immunized with live vaccine MDRV regulated the expression level of miRNAs in hepa-tocyte exosomes, which were involved in liver metabolism and other functions. The results of this study will provide a theoretical basis for the scientific prevention and control of MDRV infection.
万方数据
维普
