Isolation, identification and drug sensitivity test of Klebsiella pneumoniae strain from Rhinopithecus oxellana
A Gram-negative bacterium was isolated from the lung, heart, liver and other diseased tissues of acute dead golden monkey (Rhinopithecus oxellana). Culture and morphological observation of this bacterium were found to coincide with K. pneumoniae charac-teristics. The 16S rRNA gene of this strain was amplified by PCR with a specific DNA fragment approximately 1433 base pairs long and BLAST using the NCBI database that showed 99.7％ nucleotide sequence similarity to the human K. pneumoniae substrain 01A030 (reference sequence NR_134062.1). Ten virulence genes related to the pathogenicity of K. pneumoniae were selected, and eight virulence genes (rmpA, fimH, waG, uge, kfu, aerobactin, ureA, khe) were tested by PCR, combined with positive strain drawing test, one hvKP was successfully isolated. The results showed that the hvKP isolate was resistant to the first and third generation cephalosporins, completely resistant to lincoamide clindamycin and sensitive to afloxacin, such as ofloxacin, enrofloxacin and ciprofloxacin.
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维普
