This experiment explored the parameters of ST cell suspension culture and the reproductive process of classical swine fever virus using a bioreactor.The process was optimized from the culture medium,cells inoculation concentration and viral vaccination dose,and the whole suspension culture process of classical swine fever virus was established.The results showed that when the cell inoculation concentration was 1×106/mL and the MOI of virus inoculation was 0.05,and the medium 1 was used,the viral titer reached 107.5FAID50/mL.The process was validated twice,and the results met the requirements,which were stable and reliable,laying the foun-dation for large-scale suspension culture of classical swine fever vaccine industrialization.
关键词
ST细胞/猪瘟病毒/悬浮培养
Key words
ST cells/Classical swine fever virus/Suspension culture
维普
万方数据