Study on the optimal harvest time of porcine transmissible gastroenteritis virus cultured in microcarrier bioreactor
The purpose of this study was to investigate the effect of using microcarrier bioreactor to culture transmissible gastroenteritis virus(TGEV),and to compare the antigen titers cultured at different time periods,so as to determine the optimal harvest time of TGEV culture,improve the antigen titer,and lay the foundation for the final production of high-quality and stable live TGEV vaccine.The amount of cells reached 1.2×109 each time when the bioreactor cells were put into the tank,and the cells were cultured under the same conditions.When the cells on the microcarrier grew to a dense monolayer,the cells were inoculated with the virus,and the sam-ples were taken at 4 hours(h)after inoculation,then the samples were retained every 2 h,and the samples were taken every 1 h after 18 h,the sampling was finished until the total shedding area of the cells on the carrier reached 80%～85%,then the antigen titer were detected.The results showed that the virus titer reached the peak at 20 h to 21 h after inoculation,so it was determined that 20～21 h after inoculation was the best harvest time for the virus.
万方数据
维普
