Exploring the Regulatory Role of circRNA in Oral Squamous Cell Carcinoma Based on Bioinformatic Analysis
Objective To identify differentially circRNA in oral squamous cell carcinoma (OSCC) using bioinformatics and qRT-PCR,and to investigate the role of the circRNA-miRNA-mRNA regulatory network in OSCC. Methods Two circRNA expression datasets of OSCC were acquired from the gene expression omnibus (GEO)database. Then,differentially expressed circRNA in OSCC were screened using R software. The candidate circRNA were obtained by intersecting the screened circRNA. The expression levels of the candidate circRNA were validated in human oral keratinocyte (HOK)and OSCC cell lines by qRT-PCR. The miRNA that bind to differentially expressed circRNA were predicted with circInteractome and circBank software. Target mRNA prediction of miRNA was performed by using miRDB and TargetScan software. The target genes were subjected to GO function and KEGG enrichment analysis by DAVID. The STRING database and Cytoscape software identified the hub genes,and their relationship with prognosis was analyzed using the GEPIA survival analysis website. Results Our data identified 3 differentially expressed circRNA,7 miRNA,and 2575 potential target mRNA. GO and KEGG analysis demonstrated that the target genes were potentially implicated in OSCC pathogenesis. Three hub genes (EGF,STAT5A,LEF1)were screened out based on enrichment analysis,protein inter-action network construction,and the results of survival analysis of hub genes. The the OSCC-related circRNA-miRNA-hub genes regulatory network was constructed. Conclusions Our study provided refer-ence and broadened the thinking for the study of circRNA in the pathogenesis and treatment of OSCC.
万方数据
维普
