The Value of Serum Long Non-coding RNA XIST and MiR-150-5p in Early Prediction of Sepsis Complicated with Acute Lung Injury
Objective To investigate the early predictive value of serum long non-coding RNA XIST(lncRNA XIST)combined with microRNA(miR)-150-5p for the development of sepsis patients complicated with acute lung injury(ALI).Methods A total of 102 septic patients admitted to the Mengchao Hepatobiliary Hospital of Fujian Medical University from September 2022 to January 2024 were enrolled as the research subjects.The patients were divided into an observation group of 32 cases(sepsis complicating ALI)and a sepsis control group of 70 cases(sepsis without complicating ALI)according to the oxygenation index at 48 hours after admission;30 cases of healthy medical checkups in the same period of time were selected as the healthy control group.Basic clinical data of each group were collected,and acute physiology and chronic health status Ⅱ(APACHE Ⅱ)score of all patients were recorded.The expression levels of serum lncRNA XIST and miR-150-5 p were detected by qRT-PCR.The differences between groups were compared,the correlation between the main indicators was analyzed,the influencing factors of septic patients complicating ALI were analyzed by binary logistic regression analysis,and the early predictive value of lncRNA XIST,miR-150-5p and their combination for the development of ALI in septic patients was evaluated by receiver operating characteristics(ROC)curve respectively.Result Compared with healthy control group,the relative expression level of serum lncRNA XIST in observation group and sepsis control group was increased,and the relative expression level of miR-150-5p was decreased,and the changes were more obvious in observation group,and the differences were statistically significant(P＜0.01).Serum lncRNA XIST and miR-150-5p had a statistically significant correlation with APACHE Ⅱ scores(r=0.518,r=-0.492,P＜0.01).Binary logistic regression analysis showed that high APACHE Ⅱ score,increased lncRNA XIST relative expression level,and decreased miR-150-5p relative expression level were independent risk factors for sepsis patients complicated with ALI(P＜0.05).The area under the curve(AUC)of lncRNA XIST and miR-150-5p in predicting sepsis patients complicated with ALI were 0.725(95％CI:0.628-0.809)and 0.706(95％CI:0.608-0.792),respectively.The AUC of the combined prediction was 0.916(95％CI:0.844-0.962),which was better than the single prediction efficacy,and had higher sensitivity(96.9％)and specificity(81.4％).Conclusion Serum lncRNA XIST combined with miR-150-5p has certain clinical value in predicting sepsis patients complicated with ALI,and can be used as an early warning indicator for patients.
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