目的 探讨miRNA-29b(miR-29b)及膜联蛋白A2(ANXA2)在子宫内膜癌(EC)组织中的表达及其与预后的关系.方法 收集72例EC(EC组)、40例子宫内膜上皮瘤样病变(EIN组)和30例子宫良性疾病(对照组)患者的子宫内膜组织,检测各组miR-29b和ANXA2的表达水平,采用Pearson相关分析探索二者的相关性.根据miR-29b及ANXA2表达的中位值,将EC患者分为miR-29b低表达组、miR-29b高表达组和A NX A 2低表达组、ANXA2高表达组,采用x2检验分析不同分组间临床病理特征的差别,采用Kaplan-Meier法和Cox回归分析其与术后总生存期(OS)和无进展生存期(PFS)的关系;双荧光素酶报告基因实验验证miR-29b与ANXA2的靶向关系.结果 与对照组和EIN组比较,EC组子宫内膜组织中miR-29b的表达水平明显降低,ANXA2的表达水平明显升高(P＜0.05);miR-29b与ANXA2表达呈负相关(r=-0.321,P＜0.05).高miR-29b组患者的OS和PFS 高于低 miR-29b 组(P＜0.05),而高 ANXA2 组患者的 OS 和 PFS 低于低 ANXA2 组(P＜0.05);miR-29b高表达是EC患者OS的保护因素(HR:0.177,95％CI:0.064～0.492,P＜0.05),而ANXA2高表达是EC患者PFS的危险因素(HR:2.281,95％CI:1.139～4.567,P＜0.05).双荧光素酶报告基因实验证实A NXA2是miR-29b的靶基因.结论 miR-29b可能通过靶向负调控ANXA2的表达参与EC的进展,二者均可作为EC患者潜在的预后指标.
Expression and Clinical Significance of miRNA-29 b and Annexin A2 in Endometrial Carcinoma
Objective To investigate the expression of microRNA-29b(miR-29b)and annexin A2(ANXA2)in endometrial carcinoma(EC)tissues and their relationship with prognosis.Methods Endo-metrial tissues of 72 EC patients(EC group),40 endometrial intraepithelial neoplasia(EIN)patients(EIN group)and 30 patients with benign uterine diseases(control group)were collected,the expression levels of miR-29b and ANXA2 in each group were detected,and the correlation between them was analyzed by Pearson correlation analysis.According to the median expression of miR-29b and A NX A 2,EC patients were divided into low miR-29b expression group,high miR-29b expression group,low expression group and high expression group of ANXA2,and the clinicopathological characteristics of different groups were analyzed by x2test.Kaplan-Meier method and Cox regression were used to analyze its relationship with postoperative overall survival(OS)and progression-free survival(PFS).Dual luciferase reporter gene assay was used to verify the targeting relationship between miR-29b and ANXA2.Results Compared with the control group and EIN group,the expression level of miR-29b in the endometrial tissue of the EC group was significantly decreased,while the expression level of ANXA2 was significantly increased(P＜0.05),the expression of miR-29b was negatively correlated with ANXA2(r=-0.321,P＜0.05).OS and PFS in the high miR-29b group were higher than those in the low miR-29b group(P＜0.05),while OS and PFS in the high ANXA2 group were lower than those in the low ANXA2 group(P＜0.05).The high expression of miR-29b was a protective factor for OS in EC patients(HR:0.177,95％CI:0.064-0.492,P＜0.05),while the high expression of ANXA2 was a risk factor for PFS in EC patients(HR:2.281,95％CI:1.139-4.567,P＜0.05).Dual luciferase reporter assay confirmed that A NXA2 was the target gene of miR-29b.Conclusion miR-29b may be involved in the progression of EC by negatively regulating the expression of ANXA2,both of which can be used as potential prognostic indicators in EC patients.
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