The Impact of Luteinizing Hormone Levels on Endometrial Receptivity in Antagonist Protocol
Objective It is aimed to investigate the impact of luteinizing hormone(LH)levels on endometrial receptivity during the ovarian stimulation process by applying gonadotropin-releasing hormone antagonist(GnRH-ant)protocol for in vitro fertilization/intracytoplasmic sperm injection-embryo transfer(IVF/ICSI-ET)cycles.Methods Ninety-six infertile patients who underwent IVF/ICSI-ET with em-bryo freeze-all strategy were selected for this study.The patients were divided into groups in ascending order of LH levels at three time points:the day of antagonist administration,1-2 days after antagonist administration,and the trigger day.Endometrial tissue samples were obtained on day 5-7 after the trig-ger day,and histological examination using Hematoxylin and Eosin(H-E)staining and immunohistochemical staining using the streptavidin-peroxidase(SP)method were performed to analyze the expression of endom-etrial receptivity markers,vascular endothelial growth factor(VEGF),and leukemia inhibitory factor(LIF).Results H-E staining of the endometrial samples suggested a secretory phase endometrium in all groups without any specific pathological conditions observed.In the implementation of the GnRH-ant protocol,the analysis of the included cases in this study showed no significant differences in the expression levels of VEGF and LIF proteins,which are markers of endometrial receptivity,in the different LH level groups at the three time points of the antagonist addition day,1-2 days after the addition,and the trigger day(P＞0.05).Conclusion In the GnRH-ant protocol,the levels of LH had no significant effect on en-dometrial receptivity during the implantation window at different time points of the antagonist administra-tion day,1-2 days after antagonist administration,and the trigger day,suggesting that the levels of LH should be dynamically monitored during treatment to keep LH levels in a controllable range,and that it is not advisable to cancel fresh embryo transfer in the cycle due to LH levels.
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