Effect and Mechanism of Strontium-Rich Water on the Exercise Ability of Aged Mice
Objective To investigate the effects and underlying mechanisms of strontium-rich water on exercise ability of aged mice.Methods A total of 16 male C57BL/6 aged mice at 20 months of age were divided into strontium-rich water group and control group,with 8 mice in each group.The strontium-rich water group was provided with strontium-rich water(6.11 mg/L)in-stead of drinking water,and the control group was given pure water.Both groups were free to drink,with water bottles being changed every two days for 8 consecutive weeks.Body weight and the weight of organs such as the heart,liver and kidney were detected.The pathological changes of heart,liver,kidney and muscles were detected by HE staining.Tension tester was used to mea-sure the muscle tension of aged mice.The autonomous activity of aged mice was detected by open field experiment.The expression of inflammatory markers TNF-α,IL-6 and IL-1β in the muscles were detected by immunohistochemistry.The levels of NF-κB were detected by Western blot in both groups.Results Compared with the control group,there was no significant effect on body weight in the strontium-rich water group(P>0.05);there was no significant effect on the weight of organs such as heart,liver,and kidney of aged mice in the strontium-rich water group(P>0.05).The intervention of strontium-rich water showed no significant changes in the pathological morphology of the heart,liver,and kidneys of aged mice,but compared with the control group,the strontium-rich water group showed better muscle cell arrangement and tightness.Compared with the control group,the average muscle tension in the strontium-rich water group significantly increased(P<0.05).The intervention of strontium rich water can significantly increase the displacement distance and average velocity of aged mice over 5 minutes,and reduce their resting time(P<0.05).After intervention with strontium-rich water,the expressions of inflammatory factors TNF-α,IL-1β and IL-6 in muscles significantly reduced(P<0.05);the expressions of key proteins NF-κB and p-NF-κB in the inflammatory signaling pathway significantly reduced(P<0.05).Conclu-sion Strontium-rich water may improve the physical exercise capacity of aged mice by inhibiting the activation of the NF-κB in-flammatory signaling pathway in muscle tissue,reducing muscle inflammation,suppressing the expression of inflammatory factors,and enhancing muscle tension and spontaneous activity in aged mice.This may be one of the mechanisms by which strontium-rich water improve the exercise ability of aged mice.
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