Objective:To explore the clinical application value of indocyanine green fluorescence imaging technique in complex laparoscopic cholecystectomy.Methods:From Feb.2019 to Mar.2023,a total of 78 patients with history of gallbladder inflammation,history of gallbladder puncture,or history of upper abdominal surgery were selected.All patients were confirmed to have gallstones and cholecystitis by preoperative enhanced CT or color Doppler ultrasound.Before or during the operation,diluted indocyanine green was injected intravenously or in the gallbladder,and cholecystectomy was performed under fluorescence laparoscopy.Results:There were no adverse reactions after indocyanine green injection in 78 patients,the bile duct was developed in 71 patients,and the average operation time was(69.89±24.17)min.The average intraoperative blood loss was(36.63±37.15)mL.There was 1 case of cystic duct variation,2 cases of gallbladder bed bile leakage and 1 case of common bile duct leakage.The average postoperative hospital stay was(4.62±1.79)d,and no bile duct injury occurred.Conclusions:The application of indocyanine green fluorescence imaging technology in complex laparoscopic cholecystectomy can help find bile duct variation and bile leakage,and avoid bile duct injury,which is safe,feasi-ble and reliable.