Preliminary Study of the Effect of Live Combined Bifidobacterium and Lactobacillus Tablets on Intestinal Microflora in MRL/lpr Mouse Model with Systemic Lupus Erythematosus Based on 16S rRNA High Throughput Sequencing Technology
Objective:Based on 16S rRNA high-throughput sequencing technology,to study the effect of Live Combined Bifidobacterium and Lactobacillus Tablets(Golden Bifid)on intestinal microbiota in MRL/lpr mouse model with systemic lupus erythematosus.Methods:Twelve female SPF grade 6-8-week-old MRL/lpr mice were randomly divided into a model control group and high-dose,medium,and low-dose groups of Golden Bifid,with three mice in each group.Three female SPF grade 6-8-week-old BALB/C mice were used as the blank control group.After three days of adaptive feeding,the high-dose,medium,and low-dose groups of Golden Bifid were treated with Golden Bifid Solution(234 μg,78 μg,26 μg)respectively.The model control group and blank control group were given an equal amount of physiological saline by gavage daily.After one week of gastric lavage,DNA was extracted from the feces of 12 MRL/lpr mice and 3 BALB/C mice.The V3-V4 region of ribosomal RNA was amplified and high-throughput sequencing was performed to obtain the raw data.The data was filtered through bioinformatics methods for quality control,and the Alpha/Beta diversity of the samples was analyzed and subsequent differential analysis was performed.Results:The Alpha diversity analysis of Chao1,ACE,Shannon,and Simpson indices showed no statistically significant differences between the five groups(P>0.05).After gastric lavage,the relative abundance of Neisseria,Pseudomonas,and Haemophilus in the high-dose,medium,and low-dose groups of Golden Bifid was significantly lower than that in the model control group(P<0.05).The relative abundance of Actinobacteriota in the medium-dose group of Golden Bifid was higher than that in the model control group,the high-dose and low-dose groups of Golden Bifid(P<0.05).Compared with the blank control group,the high,medium,and low-dose groups of Golden Bifid and the model control group showed a decrease in the relative abundance of unidentified Blautia bacteria(P<0.05).Conclusion:After gastric lavage of the high,medium,and low-dose groups of Golden Bifid,the abundance of harmful bacteria such as Neisseria,Pseudomonas,Haemophilus,and Lachnospira was downregulated.After gastric lavage of the low-dose group of Golden Bifid,the beneficial bacterium Fusobacteriota was upregulated,indicating that Golden Bifid can affect the intestinal microbiota of MRL/lpr mouse model with systemic lupus erythematosus.
万方数据
维普
