Experimental Study on the Improvement of in Osteoarthritis Cartilage Degenerationby Wutou Tang(乌头汤)from the Perspective of lncRNA NEAT1/miRNA-377-3p Regulation
Objective:To investigate the mechanism of Wutou Tang(乌头汤)in regulating the expres-sion of lncRNA NEAT1/miRNA-377-3p in osteoarthritis cartilage degeneration.Methods:Forty-eight C57BL/6 mice were randomly divided into a blank control group(12 mice)and a model group(36 mice).The Hulth method was used to prepare the osteoarthritis model.After the model was established,it was randomly divided into a model control group,a Wutou Tang group,and a glucosamine sulfate capsule group,with 12 animals in each.The osteoarthritis model was identified using safranin-fast green staining.After the intervention,Micro-CT was used to observe the changes in the morphology of the knee joint,and Real-time PCR was used to detect the changes in the levels of lncRNA NEAT1,miRNA-377-3p,MMP-9,MMP-13,ADAMTS-4,ADAMTS-5,and Aggrecan in cartilage tissues.Results:Micro-CT results show:compared with the blank control group,the joint structure of the model control group was significantly deformed,with severe narrowing of the joint space,high degree of destruction of the bone articular surface,and accompanied by osteophyte formation;compared with the model control group,the degree of joint deformation and damage in mice was significantly improved in the Wutou Tang group and the glucosamine sulfate capsule group.Compared with the blank control group,the levels of lncRNA NEAT1,MMP-9,MMP-13,ADAMTS-4,and ADAMTS-5 in the model control group were significantly increased,while the relative expression levels of miRNA-377-3p and Aggrecan were significantly decreased(P<0.05);in the Wutou Tang group,the levels of lncRNA NEAT1,MMP-9,MMP-13,ADAMTS-4,and ADAMTS-5 were significantly reduced,and the levels of miRNA-377-3p and Aggrecan were upregulated(P<0.05).Conclusion:Wutou Tang can improve cartilage degeneration in osteoarthritis,which is related to the regulation of lncRNA NEAT1/miRNA-377-3p.
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