The Effects of siRNA Targeting Nuclear Transcription Factor-κB and Methotrexate Nanoliposomes on Bone Destruction in Rheumatoid Arthritis Rats
Objective:To investigate the effects of siRNA targeting nuclear transcription factor-κB and methotrexate nanoliposomes on bone destruction in rheumatoid arthritis rats.Methods:Forty-eight healthy SD rats were randomly divided into a blank control group of 10 rats and a model group of 38 rats.Thirty successfully modeled rats were randomly divided into a model control group,an MTX group,and an MTX composite nanoliposome group(si-MTX group),with 10 rats in each group,measuring the body mass,organ index,toe swelling,serum inflammatory factor levels,as well as the expression levels of related genes and proteins in synovial tissue of rats in each group before and after treatment.Results:Both MTX and composite nanoliposomes can significantly reduce thymus index,spleen index,and plantar swelling in collagen induced arthritis rats,lower serum levels of inflammatory factors,regulate the expression of related genes and proteins in synovial tissue(P<0.05),and the si-MTX group has a stronger effect(P<0.05).Conclusion:The mechanism by which composite nanoliposomes exert their effects may be by blocking the NF-κB pathway,reducing the release of pro-inflammatory factors,inhibiting bone resorption,and protecting articular cartilage;and it has higher targeting and safety.
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