首页|Extrusion/Inkjet Printing of Verteporfin-Loaded Bilayer Skin Substitutes for Wound Healing and Structure Reconstruction

Extrusion/Inkjet Printing of Verteporfin-Loaded Bilayer Skin Substitutes for Wound Healing and Structure Reconstruction

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The shortage of transplantable skin is the leading cause of death in patients with extensive skin defect.Addressing this chal-lenge urgently requires the development of skin substitutes capable of wound repair and facilitating skin regeneration.In this study,a biomimetic bilayer skin tissue model consisting of collagen,gelatin/sodium alginate,fibroblasts,human umbilical vein endothelial cells,keratinocytes,melanocytes,and verteporfin was devised.Then,the skin model was fabricated using precise extrusion/inkjet bioprinters,and it reconstruction capabilities were evaluated through skin defect repair experiments.The printed skin tissue reduced the inflammatory response of the wound by 38％and inhibited the expression of TGF-β and YAP,and promoted the transformation of macrophages from Ml phenotype to M2 phenotype,thus promoting the reason-able reconstruction of fibronectin and collagen on the wound,finally promoting the wound healing,and reducing the wound contraction and scar formation.In addition,the proliferation and differentiation of human umbilical vein endothelial cells,keratinocytes,and melanocytes in printed skin increased the number of regenerated blood vessels by 123％,while promoting the reconstruction of multilayer epidermal structure and skin color.The outcomes of this investigation present a promising skin model and therapeutic strategy for skin injury,offering a potential avenue for the reconstruction of skin structure and function.

BioprintingMulticellular printingBiomimeticSkin substitutesWound healing

Tian Jiao、Ruilu Zhou、Junrong Jiao、Junna Jiao、Qin Lian

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School of Intelligent Manufacturing and Electrical Engineering,Nanyang Normal University,1638 Nanyang Road,Nanyang 473061,Henan Province,People's Republic of China

Nanyang Normal University Library,Nanyang Normal University,1638 Nanyang Road,Nanyang 473061,Henan Province,People's Republic of China

Key Lab for Special Functional Materials of Ministry of Education,School of Materials Science and Engineering,Henan University,Kaifeng 475004,People's Republic of China

School of Basic Medical Sciences,Xinxiang Medical University,601 Jinsui Avenue,Xinxiang 453000,Henan Province,People's Republic of China

State Key Laboratory for Manufacturing System Engineering,Xi'an Jiaotong University,28 Xianning West Road,Xi'an 710049,Shaanxi Province,People's Republic of China

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2024

10.1007/s42235-024-00585-5

仿生工程学报(英文版)
吉林大学

仿生工程学报(英文版)

CSTPCDEI
影响因子：0.837
ISSN：1672-6529
年,卷(期)：2024.21(6)