首页|铁皮石斛组培苗生根壮苗培养基的优化筛选研究

铁皮石斛组培苗生根壮苗培养基的优化筛选研究

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目的:筛选铁皮石斛组培苗生根壮苗的适宜培养基。方法在相同的培养条件下,将铁皮石斛相同高度幼苗接种于9种不同的培养基中,记录各组组培苗的生长情况;采用紫外分光光度法测定各组组培苗的多糖含量并进行综合分析。结果铁皮石斛组培苗的生根壮苗以1/2-MS+土豆泥+0.5-mg-1·L7BA+0.2-mg-1·L-NAA+活性炭+花宝1号+3%蔗糖或1/2-MS+土豆泥+0.3-mg-1·L-BA+0.15-mg-1·L-NAA+活性炭+花宝1号+3%蔗糖的培养基为佳。
Studies on optimized selection of the culture medium about7rooting and growth-promoting of Dendrobium officinale seedlings
to optimize the most appropriate culture medium for rooting and growth-promoting of Dendrobium officinale seedlings. in the same culture conditions, the same height seedlings were seeded in 9 different medium, compared the growth of the seedlings through the observation records; used UV spectrophotometry to de tect polysaccharide content of each cultivation and made the comprehensive analysis. Results and Conclusion: the best medium for rooting and growth-promoting of Dendrobium officinale Seedlings was 1/2 MS + mashed potatoes + 0.5 mg/L BA + 0.2 mg/L NAA + activated carbon + Huabao NO.1 + 3%sucrose,or, 1/2,MS,+,mashed,potatoes,+,0.3,mg/L,BA,+,0.15,mg/L,NAA,+,activated,carbon,+,Huabao,NO.1,+,3%,sucrose.

Dendrobium officinaleTissue culturepolysaccharide

李海燕、林丽婷、谢燕婷、温嘉乐、罗国荣、莫文芳、刘瑞驹

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佛山科学技术学院药学系,广东佛山528000

广东国方医药科技有限公司,广东东莞523000

铁皮石斛 组织培养 多糖

广东省大学生创新创业训练计划项目

1184712047

2015

佛山科学技术学院学报(自然科学版)
佛山科学技术学院

佛山科学技术学院学报(自然科学版)

影响因子:0.226
ISSN:1008-0171
年,卷(期):2015.(2)
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