Establishment and application of a nested PCR detection method for Bartonella
In order to develop a highly sensitive and specific detection method for Bartonella,specific primers were designed based on the conserved 16S sequence of Bartonella.By optimizing the reaction system and conditions,a nested PCR detection method was established.This method was applied to analyze 100 spleen samples collected from Rattus norvegicus in Huadu district,Guangzhou city in 2023.The results showed that the method exhibited excellent specificity,accurately detecting without any cross-reactivity with control strains of Coxiella burnetii,Anaplasmataceae,Leptospira,Rickettsia and Borrelia.Moreover,the method displayed high sensitivity,with a minimum detection limit of 7.66×101 copies·μL-1.Furthermore,the established method successfully detected four different Bartonella species in 100 clinical samples,including one species associated with human diseases.The nested PCR method for Bartonella detection demonstrated specificity,sensitivity,and reproducibility,providing valuable technical support for the detection of Bartonella in clinical samples.
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