Effects of RNA Interference Mediated Gene Silencing of Hypoxia Inducible Factor-1α on the Cell Proliferation and Cell Cycle of the Human Pancreatic Cancer Patu8988 Cells
Objective To investigate the influence of RNA interference on expression of HTF-lα gene on the cell proliferation and cell cycle of the human pancreatic cancer cell line Patu8988. Methods Construct the lentiviial vector-mediated RNA interference of HTF-1α Patu8988 cells transfected with the empty vector served as hypoxia negative control group, Patu8988 cells not transfected with vector served as hypoxia blank control group, cells cycle and cell proliferation were evaluated by using flow-cytometric anlysis and MTT assay. Results The growth of the pancreatic cancer cell was decreased obviously compared to the blank control group and negative control group (F = 58.58, P =0.000; F=55.91, P =0.000); In addition, the percentage of cells in G0/G1 phase in experimental group was significantly more than that in blank control group (t= 10.87, P = 0.01), the percentage of cells in S and G2/M phase in experimental group were less than that in blank control group(t = 6.44, P=0.05;l =5.49,P =0.005). Conclusion Gene silencing of HDT-lα using RNAi lentiviral vector-mediated RNA can inhibit the expression of HIF-lα, inhibit the proliferation and cell cycle at GD/G1 phase, decrease the growth of the Patu8988 pancreatic cancer cells in vitro.
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