Objective To evaluate the correlation, linear range and detection effect of sandwich and competitive chenulumines-cence immunoassay for the detection of hyaluronic acid from human serum the performance of the 2 methods was compared. Methods The level of hyaluronic acid in 197 sera was detected by the sandwich and competitive chemiluminescence immunoassay, and the results were analyzed statistically. The precision, recovery and sensitivity of the 2 methods were assessed simueltaneously. Results The accuracy and precision of sandwich chemiluminescence immunoassay were better than competitive chemiluminescence immunoassay. The detection limit of sandwich chemiluminescence immunoassay could leach 0.66 μg/L and the competitive chemiluminescence im-munoassay just could reach 7.26 μg/L. However, both methods could exactly detect hyaluronic acid from serum. The correlation of them was y = 1.1893χ-25.974(r =0.9871), whose results showed no significant difierence(t = 1.966,P =0.664). Conclusion Sandwich and competitive chemiluminescence immunoassay could all exactly detecting hyaluronic acid concentration of serum, but sensitivity, repeatability and recovery of sandwich method were better than those of competitive method's.
NETL
NSTL
维普
万方数据
