RA患者血清TGF-β1、MMP-3、IL-18和CRP测定的临床意义
Clinical Significance of Determination of Serum TGF-β1, MMP-3,IL-18 and CRP Levels in Patients with Rheumatoid Arthritis
荆炳霞 1朱静 2张晓懿2
作者信息
- 1. 江苏省常熟市医学检验所,215500
- 2. 扬州大学第五临床医学院常熟二院核医学科,215500
- 折叠
摘要
目的:探讨类风湿性关节炎(rheumatoid arthritis,RA)患者相关血清炎症介质和免疫指标水平的变化及意义.方法:本文分别采用放射免疫分析、酶联免疫法、免疫比浊法检测RA患者血清转化生长因子β1(transforming growth factor-β1,TGF-β1)、基质金属蛋白酶-3(matrixmetalloproteinase- 3,MMP-3)、白细胞介素-18(interleukin-18,IL-18)及C-反应蛋白(C-reactive protein,CRP)水平,并分析其水平的变化与临床病情活动程度的关系.结果:IL-18水平缓解期组和活动期组两组均非常显著地高于正常对照组(P<0.01);TGF-β1水平则显著高于正常对照组(P<0.05);MMP-3水平活动期组和缓解期组两组较正常对照组差异具显著性(P<0.01,P<0.05);CRP水平缓解期与正常对照组比较无显著性差异(P>0.05),而活动期组则显著高于正常对照组(P<0.05).结论:RA患者血清TGF-β1、MMP-3、IL-18 和CRP可能参与了RA的发病机制的发生及病情进展过程,该类测定对于了解病情和预后有一定帮助.
Abstract
Objective To explore the clinical significance of changes on serum TGF-β1, IL-18, MMP-3 and CRP levels in patients with rheumatoid artliritis(RA). Methods 80 patients with RA were divided into 2 groups: ① stable stage group (n =40) , ② active stage group(n =40), 40 healthy people served as control groups. The serum TGF-β1( with RIA) , IL-18, MMP-3 (with ELISA) and CRP(with immunoturbiodimetry) levels were determined in 80 patients with RA and 40 controls. Results The serum level of IL-18 was significantly higher in stable stage group and active stage group than that in control groups (all, P<0.01). The serum level of TGF-β1, was evidently higher in stable stage group and active stage group than that of control groups (all,P <0.05). The serum level of MMP-3 was obviously higher in both the stable stage and active stage group than the controls (P<0.05,P<0.01). The serum level of CRP showed a minor changes in stable stage group than the controls (P >0.05) , but the serum level of CRP has risen significantly inactive stage group than that in control group (P<0.05). Conclusion TGF-β1, IL-18, MMP-3 and CRP might to play important roles in the pathogenesis and the coumse of rheumatoid arthritis. Therefore, determination of which may serve as a predictor to realize and evaluate of RA.
关键词
类风湿性关节炎/白细胞介素-18/转化生长因子β1/基质金属蛋白酶-3/C-反应蛋白Key words
rheumatoid arthritis(RA)/interleuiin-18(IL-18)/transforming growth factor-β1 (TGF-β1 )/metalloproteinases-3 (MMP-3)/C-reactive protein(CRP)引用本文复制引用
出版年
2013
NETL
NSTL
维普
万方数据