乳腺癌患者手术前后血清IL-2、MMP-9、TK1和sFas测定的临床价值
Clinical Value of Determination on Serum IL-2,MMP-9,TK1 and sFas Levels both Before and After Operation in Patients with Breast Cancer
谢国强 1肖华龙 1邵耀明 1马坚1
作者信息
- 1. 江苏省无锡市人民医院检验科,214000
- 折叠
摘要
目的:讨论乳腺癌患者血清IL-2、MMP-9、TK1和sFas四项指标在手术治疗前后测定的临床价值.方法:血清IL-2采用放射免疫分析;MMP-9和sFas采用酶联免疫吸附分析法检测;TK1测定采用CIS-型免疫印迹-增强发光检测系统检测.结果:本文结果显示,血清IL-2水平对照组与正常组无显著差异(P>0.05),术前组分别低于对照组和正常组(P<0.05,P<0.01),术后组较术前组升高显著(P<0.05).MMP-9水平则术前组和对照组均显著高于正常组(P<0.05,P<0.01),术后组较术前组下降显著(P<0.05),但仍显著高于正常组(P<0.05).TK1水平术前组和对照组均显著高于正常组(P<0.05,P<0.01),同时术前组又显著高于对照组(P<0.01),术后组较术前组下降显著(P<0.05),但仍显著高于正常组(P<0.05).sFas水平的变化同MMP-9.结论:患者血清4项指标手术前后发生了显著变化有可能与乳腺癌的发生和病理过程有关,其测定对于了解其病理机制和评估预后有一定帮助.
Abstract
Objective To discuss clinical value of detennination of serum IL-2,MMP-9,TK1 and sFas levels before and after operation in patients with breast cancer. Methods Serum level of IL-2 was determined with RIA, serum levels of MMP-9 and sFas were determined with EIA. Serum level of TK1 was determined with GIS-1 immune blot enhanced chemiluminescence detection system. Re-SllltS Postoperative patients with breast cancer the serum level of IL-2 showed significantly increased than that in patients before operation (P<0.01) , before operation the serum level of IL-2 proved significantly lower than that in the controls and healthy woman( all P <0.05). In postoperative patients group,the serum level of MMP-9 expressed significantly dropped than that of patients before operation (P < 0.05) , before operation the serum level of MMP-9 was significantly higher than that in the controls and healthy woman( all P <0.05) . In postoperative patients group,the serum level of TK1 appeared significantly dropped than preoperative(P<0.05) , before operation the serum level of MMP-9 presented significantly higher than that in controls and healthy woman( all P < 0.05 ). The changes of serum levels on sFas as well as in MMP-9. The results of correlative analysis showed, the serum level of TK1 with MMP-9 and sFas levels proved mutually positively correlated (r=0.548,P<0.01;r=0.241,P<0.05) , while the serum level of TK1 with IL-2 level presented positively correlation(r= -0.221 ,P<0.05). Conclusion The changes of 4 serum indexes levels were related with the development of breast cancer .the determination might be helpful for pathogenesis and prediction.
关键词
乳腺癌/白细胞介素-2/基质金属蛋白酶9/胸苷激酶1/可溶性凋亡相关因子Key words
breast cancer/interleukin-2/matrix metalloptoteinase/thymidine kinasel/soluble factor associated suicide引用本文复制引用
出版年
2013
NETL
NSTL
维普
万方数据