亚低温治疗对心肺复苏后大鼠脑组织损伤保护作用的研究
Investigation of Protective Effects of Mild Hypothermia Treatment on Rats Brain After Cardiopulmonary Resuscitation
陈玉熹 1缪心军 1陈红辉 1陈之力 1尤荣开1
作者信息
- 1. 浙江省温州市中心医院急诊科,325000
- 折叠
摘要
目的:探讨亚低温治疗对心肺复苏大鼠脑组织的保护作用及其在临床应用中的意义.方法:50只SD雄性大鼠随机分为对照组和实验组,大鼠经心跳停搏后行心肺复苏手术(CPR),待自主循环恢复24h后,分别检测脑组织中神经元特异性烯醇化酶(NSE)基因水平及血清中NSE蛋白水平,并采用干湿法检测各组大鼠脑组织含水量.结果:与对照组相比,实验组血清中NSE蛋白水平明显升高,脑组织中NSE mRNA水平无明显变化.4min停搏组含水量(81.7±2.1)%,较对照组(75.6±1.7)%显著增加(P<0.01).亚低温保护组(76.9±1.9)%较实验组显著减少(P<0.01).结论:亚低温治疗可以有效减轻心肺复苏后大鼠脑组织的损伤程度.
Abstract
Objective To explore the protective effects of mild hypothermia treatment on rats brain after caidiopuhnonary resuscitation and its clinical application. Methods Fifty SD male rats were randomly divided into control and experimental groups. Car-diopulmonary resuscitation (CPR) was implemented after cardiac arrest in rats. The gene expression level of neuron-specific enolase ( NSE) in brain and the protein level of NSE in serum were measured 24 hours after restoration of spontaneous circulation. The brain water content was also evaluated with dry-wet weight method. Results Compared with the control group, the experimental group s serum NSE protein level was increased obviously, whereas no significant change in the NSE mRNA level in brain tissues was observed. The water content of the 4 min arrest group(81.7 ±1.7)% increased markedly compared with the control group(74.6 ±1.4)% , P< 0.01. The water content of mild hypothermia protection group(76. 9 ± 1. 9)% reduced distinctly compared with the experimental group, P <0.01. Conclusion Mild hypothermia therapy can significantly extenuate the brain damage of rats which after cardiopulmonary resuscitation.
关键词
亚低温治疗/心肺复苏/脑损伤/神经元特异性烯醇化酶(NSE)Key words
mild hypothermia treatment/cardiopulmonary resuscitation/brain injury/neuron-specific enolase (NSE)引用本文复制引用
出版年
2013
NETL
NSTL
维普
万方数据