抗中性粒细胞胞浆抗体检测在自身免疫性疾病中的应用
The Application of Antineutrophil Cytoplasmic Antibodies Detection in Autoimmune Diseases
蔡枫 1谷丽华 1夏莉婷1
作者信息
摘要
目的:探讨抗中性粒细胞胞浆抗体(ANCA)在自身免疫性疾病中的应用.方法:用间接免疫荧光法(IIF)、免疫印迹法(WB)和酶联免疫吸附法(ELISA)检测了747例自身免疫性疾病患者血清.结果:系统性红斑狼疮(SLE)患者ANCA阳性率为24.47%(105/429),干燥综合征(SS)为13.15%(15/114),类风湿关节炎(RA)为8.64%(7/81).混合性结缔组织病(MCTD)为14.28%(8/56).样本量较少的皮肌炎(DM)、强直性脊柱炎(AS)、自身免疫性肝炎(AIH)等及40例健康对照者ANCA均为阴性.从p-ANCA阳性、MPO阴性选取了36份诊断为SLE患者血清进行ANCA 其他6种相关的抗中性粒细胞胞浆抗体确认试验.结果:4例为乳铁蛋白阳性,1例为杀菌性/通透性增强蛋白阳性.结论:ANCA在自身免疫性疾病中有一定的阳性检出率,阳性靶抗原以MPO 和PR3 为主要的靶抗原.部分SLE患者中有其他抗原LF及BPI的检出,提示该特异性靶抗原可能与炎症过程相关.
Abstract
Objective To explore the role of anti-neutrophil cytoplasmic antibody ( ANCA) in the diagnosis of autoimmune diseases. Methods ANP.A in the serun of 747 autoimmune diseases patients were detected by indirect immumoflurescence (IIF),Western blot technique( WB) and ELBA . Results Systemic lupus erythematosus( SLE) patients with ANCA-positive rate was 24.47% (105/429). The positive rate of ANCA was 13.15% with Sjogren' s Syndrome. Rheumatoid arthritis was 8. 64% (7/81). Mixed connective tissue disease was 14.28% (8/56). Small sample size dermatomyositis, ankylosing spondylitis, autoimmune hepatitis and 40 healthy controls all the cases showed ANCA negative. From p-ANCA positive, of MPO-negative selected 36 diagnostic ANCA other six kids of anti-neutrophil cytoplasmic antibody confirmatory test for the serum of patients with SLE .Four case positive for lactoferrin ( LF) , one cases of bactericidal / permeability-increasing protein( BPI) positive. Conclusion ANCA presented definite positive detection rate in autoimmune diseases. Positive target antigens MPO and PR3, the main target antigen. Some patients with SLE also could detect other antigen such as LF and BPI. Suggesting that the specific target antigen might be related to the inflammatory process.
关键词
抗中性粒细胞胞浆抗体/髓过氧化物酶/蛋白酶3/自身免疫性疾病Key words
anti-neutrophil cytoplasmic antibody/myeloperoxidase (MPO)/proteinase3(PR3)/autoimmune diseases引用本文复制引用
出版年
2013
NETL
NSTL
维普
万方数据