CLIA测丙肝抗体合并EIA测丙肝核心抗原作为丙肝感染初筛实验的探讨
The Study of Screening Test Using Anti-HCV CLIA and HCV-cAg EIA in HCV Infection1
王燕 1窦恒利 2尹秋霞 1徐军1
作者信息
- 1. 济南军区总医院实验诊断科,250031
- 2. 济南市立四院,250031
- 折叠
摘要
目的:比较CLIA和EIA检测抗-HCV分别合并ELISA测定总HCV-cAg对诊断HCV感染的检出率,分析CLIA抗-HCV联合EIA法HCV-cAg作为HCV感染初筛试验的重要临床意义.方法:分别用CLIA、EIA检测所有临床标本中的抗-HCV和HCV-cAg,选取抗-HCV初筛阳性样本进行HCV-RNA和ALT检测.结果:6461例样本中,EIA和CLIA检测抗-HCV阳性率分别为2.86%和3.17%,anti-HCV(+)/HCV-cAg(-)组,CLIA检出患者ALT含量与EIA检出患者无显著性差异.而HCV-RNA阳性率(98.8%)显著高于EIA检出患者(91.7%),P<0.05;anti-HCV(+)/HCV-cAg(+)组,CLIA检出患者ALT含量与EIA法检出患者无显著差异.而HCV-RNA阳性率(97.8%)显著高于EIA检出患者(86.5%),P<0.05.结论:用CLIA检测抗-HCV联合EIA法HCV-cAg进行HCV感染初筛实验,与传统EIA法抗-HCV、HCV-cAg初筛试验相比,对HCV感染患者阳性检出率更高、降低假阳性率更低、与HCV-RNA测定结果阳性符合率高,检查结果提示,可作为临床对HCV感染患者早期诊断和治疗的有效检验程序.
Abstract
Objective To compare the detection rate of anti-HCV with chemiluminescence immunoassay(CLIA) and EIA, and analyze the clinical significance of anti-HCV CLIA and HCV-cAg ELISA in diagnosis of HCV infection. Methods CLIA and EIA were respectively performed to screen for anti-HCV, and HCV-cAg. Positive results of inital screening were followed up with ALT and HCV-RNA. Results In 6461 patient samples, with the CLIA method 3.17% of patients and with the EIA method 2.86% had test results for anti-HCV. In anti-HCV( + )/HCV-cAg(-) patients, no significant difference about ALT level was observed between the anti-HCV positive patients using CLIA or EIA, but significant difference about HCV-RNA was observed between the anti-HCV positive patients u-sing CLIA (98.7% ) and EIA (94.6% ) individually, P <0.05. In anti-HCV( + )/HCV-cAg( + ) patients, HCV-RNA positive rate of patients detected by CLIA (97.8% ) presented significantly higher than by EIA(86.5% ) , P < 0.05. Conclusion Comparing with anti-HCV and HCV cAg by EIA, combining anti-HCV CLIA with HCV-cAg EIA provides higher detection rate , lower false-positive rate and good consistency with the detection results of HCV-RNA. The findings indicated, it is effective laboratory procedures for early diagnosis and treatment of hepatitis C virus infection.
关键词
丙型肝炎病毒/丙型肝炎抗体/丙型肝炎核心抗原/化学发光免疫测定Key words
hepatitis C virus/anti-hepatitis C virus/hepatitis C virus core antigen/chemiluminescent immunoassay引用本文复制引用
出版年
2013
NETL
NSTL
维普
万方数据