Objective To compare the detection rate of anti-HCV with chemiluminescence immunoassay(CLIA) and EIA, and analyze the clinical significance of anti-HCV CLIA and HCV-cAg ELISA in diagnosis of HCV infection. Methods CLIA and EIA were respectively performed to screen for anti-HCV, and HCV-cAg. Positive results of inital screening were followed up with ALT and HCV-RNA. Results In 6461 patient samples, with the CLIA method 3.17% of patients and with the EIA method 2.86% had test results for anti-HCV. In anti-HCV( + )/HCV-cAg(-) patients, no significant difference about ALT level was observed between the anti-HCV positive patients using CLIA or EIA, but significant difference about HCV-RNA was observed between the anti-HCV positive patients u-sing CLIA (98.7% ) and EIA (94.6% ) individually, P <0.05. In anti-HCV( + )/HCV-cAg( + ) patients, HCV-RNA positive rate of patients detected by CLIA (97.8% ) presented significantly higher than by EIA(86.5% ) , P < 0.05. Conclusion Comparing with anti-HCV and HCV cAg by EIA, combining anti-HCV CLIA with HCV-cAg EIA provides higher detection rate , lower false-positive rate and good consistency with the detection results of HCV-RNA. The findings indicated, it is effective laboratory procedures for early diagnosis and treatment of hepatitis C virus infection.
hepatitis C virusanti-hepatitis C virushepatitis C virus core antigenchemiluminescent immunoassay
NETL
NSTL
维普
万方数据
