初诊鼻咽癌患者外周血CD4+CD25+Foxp3+调节性T细胞的变化及与TGF-β1的相关性
The Changes of CD4+CD25+Foxp3+ Regulatory T Cell in Untreated Nasopharyngeal Carcinoma Patients of Peripheral Blood and the Correlativity with TGF-β1
汪铁军 1谢中华 1郑元秀 1黄芳芳 1王珠美1
作者信息
- 1. 浙江省杭州市余杭区第二人民医院,311121
- 折叠
摘要
目的:观察初诊鼻咽癌患者外周血CD4+CD25+Foxp3+调节性T细胞的变化及与TGF-β1的相关性.方法:采用流式细胞术检测30例正常对照者(对照组)及47例初诊鼻咽癌患者(患者组,临床TNM分期Ⅰ期11例、Ⅱ期16例、Ⅲ期12例、Ⅳ期8例)外周血单个核细胞CD4+CD25+Foxp3+调节性T细胞数目,并计算CD4+CD25+Foxp3+调节性T细胞占CD4+T淋巴细胞的百分比;酶联免疫吸附试验法(ELISA)检测其血清TGF-β1水平,分析CD4+CD25+Foxp3+调节性T细胞与TGF-β1的相关性.结果:与正常对照组比,患者组PBMC CD4+CD25+Foxp3+Treg占CD4+T淋巴细胞的百分比、CD4+CD25+Foxp3+Treg绝对数及血清TGF-β1水平均显著升高,差异有显著的统计学意义(P<0.01,P<0.05).相关性分析表明,CD4+CD25+Foxp3+Treg/CD4+T及CD4+CD25+Foxp3+Treg绝对数均与血清TGF-β1水平呈明显正相关(r分别为0.737、0.679,P<0.01).结论:初诊鼻咽癌患者外周血TGF-β1及CD4+CD25+Foxp3+Treg增高,TGF-β1可能参与诱导CD4+CD25+Foxp3+Treg的生成,与CD4+CD25+Foxp3+Treg相互作用,从而发挥免疫抑制作用.
Abstract
Objective To observe the changes of CD4+CD25 + Faxp3+ regulatory T cell in untreated nasophaiyngeal carcinoma patients of peripheral blood and the relationship between CD4+ CD25+ Fozp3+ regulatory T cell with the TGF-β1. Methods The CD4 + CD25 + Faxp3+ regulatory T cell of peripheral blood in 47 patients with untreated nasopharyngeal carcinoma (TOM Ⅰ11,Ⅱ16,Ⅲ 12, Ⅳ8) and 30 normal healthy persona served as control group. All of patients and controls was detected by flow-cytometry. Ihe level of serum TGF-β1 was detected by ELISA. The relationship between the proportion and number of CD4+CD25 + Foxp3+ regulatory T cell with TGF-β1 were statistically analyzed. Results Compared with control group, the proportion and number of CD4+ CD25+ Foxp3 + regulatory T cell and TGF-β1 in nasopharyngeal carcinoma patients of peripheral blood were increased significantly ( P < 0. 01, P < 0. 05). The proportion and number of CD4+CD25+ Fozp3+ regulatory T cell were postive correlated with the level of serum TGF-β1. Conclusion The level of TGF-β1, the proportion and number of CD4+ CD25+ Foxp3+ regulatory T cell were increased in untreated nasopharyngeal carcinoma patients of peripheral blood. TGF-β1 may play an important role in generation of CD4+ CD25 + Foxp3 + regulatory T,which interacting with CD4+CD25 + Foxp3+ regulatory T and in result to exert of immunosuppression.
关键词
鼻咽癌/转化生长因子-β1/调节性T细胞/流式细胞术Key words
nasopharyngeal carcinoma/transforming growth factor-β1(TGF-β1)/regulatory T cell/flow cytometry引用本文复制引用
出版年
2013
NETL
NSTL
万方数据