老年COPD患者血清SP-D、NE、IL-8和α-HBD水平的变化与病情进展的关系
The Relationship of the Changes of Serum SP-D, NE, IL-8,α-HBDH Levels and Progress of the Disease in Elderly Patients with COPD
王卓 1王滨 1洪侃 1万鹤鸣 1郁洁 1陆守荣1
作者信息
- 1. 江苏省无锡市人民医院老年医学科,214023
- 折叠
摘要
目的:探讨血清四项指标浓度的变化与慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)患者病情进展的关系和临床价值.方法:血清肺泡表面活性蛋白D(surfactant protein D,SP-D),中性粒细胞弹性蛋白酶(neutronphil elastase,NE)采用酶联免疫法(ELISA)检测,白细胞介素8(interleukin 8,IL-8)含量则采用放射免疫分析(RIA),α-羟丁酸脱氢酶(alpha-hydroxybutyric dehydrogenase,α-HBDH)测定采用全自动生化分析法(ACA).结果:临床及测定结果:两组间除性别和平均年龄两项临床记录指标外,余五项指标均存在显著性差异(P<0.05,P<0.01).血清指标中SP-D水平Ⅰ级组较正常对照组略升高,但无显著性差异(P>0.05),Ⅱ、Ⅲ级组水平则均显著高于正常对照组(P<0.05,P<0.0),患者分级组间比较也存在显著性差异(P<0.05).IL-8水平则于Ⅰ级组已显著高于正常对照组(P<0.05),Ⅱ、Ⅲ级组水平则均显著高于正常对照组(P均<0.01),患者分级组间比较也存在显著性差异(P均<0.05).NE水平的变化统计结果同IL-8.α-HBDH活性的变化Ⅰ、Ⅱ级两组较正常对照组皆无显著统计学差异(P均>0.05),Ⅲ级组水平则显著高于Ⅱ级组和正常对照组(P<0.05,P<0.01).相关分析结果:血清指标与患者呼吸功能指标FEV1的相关性分析显示,FEV1 与SP-D水平Ⅰ级组无显著相关性(r=-0.26,P>0.05),Ⅱ级组呈弱相关(r=-0.36,P=0.05),Ⅲ级组则呈显著负相关(r=-0.76,P<0.05).FEV1 与IL-8水平Ⅰ级组呈显著负相关(r=-0.55,P<0.05),Ⅱ级组呈显著负相关(r=-0.65,P<0.05),Ⅲ级组则呈显著负相关(r=-0.70,P<0.05).FEV1 与NE水平Ⅰ级组呈显著正相关(r=-0.60,P<0.05),Ⅱ级组呈显著负相关(r=-0.65,P<0.05),Ⅲ级组则呈显著负相关(r=-0.70,P<0.05).FEV1 与α-HBDH水平Ⅰ级组无显著相关性(r=-0.30,P>0.05),Ⅱ级组也无显著相关(r=-0.15,P>0.05),Ⅲ级组则呈弱相关(r=-0.41,P=0.05).结论:血清四项指标浓度的变化与COPD患者病情进展的关系密切;其测定对于老年COPD患者病情的了解和预后评估有一定的临床价值.
Abstract
Objective To explore the relationship and the clinical significance of changes of four serum markers (SP-D,NE,IL-8,α-HBD) in elderly patients with COPD. Methods Serum SP-D, NE levels were measured with ELISA, while IL-8 with RIA,α-HBDH with ACA. Results Clinical and measurement results; the cases and control appeared to be well matched on age and gender (as shown in table 1) , however, there proved significant statistical differences among five indicators(P<0.05,P<0.01). Serum level of SP-D in Ⅰ group showed slightly higher than that of control group, but there expressed no significant statistical difference(P > 0.05) ,Those in Ⅱ and Ⅲ group presented significantly higher than normal control group(P<0.05,P<0.01) ,there were also significant differences between those in patients with grade group( P < 0.05 ). Serum level of IL-8 in Ⅰ group indicated significantly higher than normal control group(P <0.05) ,those in Ⅱ and Ⅲ group proved markedly higher than the normal control group(P <0.01, P < 0.01) ,there were also significant differences between those in patients with grade group(P <0.05). Serum level of NE in statistical results with IL-8. Serum levels of α-HBDH in Ⅰ group and Ⅱ group proved no statistically significant differences(P >0.05) , those in Ⅲ group gained significantly higher than E group and normal control group(P<0.05,P<0.01). Correlative analysis results; analysis of serum markers and respiratory function parameters FEV1 showed that; FEY1 and serum level of SP-D no significant correlation in Ⅰ group(r= -0.26 ,P>0.05) , but slightly correlation in Ⅱ group (r = -0.36,P = 0.05) , while significantly negative correlation in Ⅲ group (r = -0. 76,P <0. 05). FEV1 and serum level of IL-8 were significantly negative correlation in Ⅰ , Ⅱ , Ⅲ group(r = -0.55,P<0.05; r= -0.65,P<0.05; r= - 0.70,P< 0.05, respectively). FEV1 and serum level of NE were significantly positive correlation in Ⅰ group(r=-0.60, P<0.05) , while those in Ⅱ, Ⅲ group were significantly negative correlation(r = -0.65,P< 0.05; r = -0.70,P <0.05, respectively). There were no significantly correlation between FEV1 and serum α-HBDH in Ⅰ ,Ⅱ group (r = -0.30, P>0.05; r= -0.15,P>0.05, respectively), slightly correlation in Ⅲ group could be found(r= -0.41,P=0.05). Conclusion The changes of serum concentrations of four indicators closely related to the progression of COPD patients, the determination of above described serum items levels provides definite clinical values to realize the disease and outcome prediction in elderly patients with COFD.
关键词
慢性阻塞性肺疾病/肺泡表面活性蛋白D/中性粒细胞弹性蛋白酶/白细胞介素8/α-羟丁酸脱氢酶Key words
COPD/SP-D/NE/IL-8/α-HBDH引用本文复制引用
出版年
2013
NETL
NSTL
维普
万方数据