125I标记单克隆抗体4E5的制备及其在小鼠体内的分布
Preparation of 125I Labeled Monoclonal Antibody 4E5 and the Biodistribution of 125I-4E5 in Normal Mice
翟士军 1邱玉华 2万卫星 1郁春景 1徐巧玲 1陈永井 2吴玉玉 3齐晓薇3
作者信息
- 1. 无锡市第四人民医院核医学科,214062
- 2. 苏州大学医学部免疫学系,215123
- 3. 无锡市第四人民医院病理科,214062
- 折叠
摘要
目的:探讨放射性核素125I标记单克隆抗体4E5的方法,观察标记物在正常小鼠体内的生物学分布.方法:采用Iodogen法进行单克隆抗体4E5的125I标记,标记产物用Sephadex G-50分离纯化,三氯醋酸(TCA)法测定标记率和放化纯,并对标记物的稳定性进行分析.取45只昆明小鼠随机分成9组,每只小鼠从尾静脉注入剂量为148KBq/0.2 ml的125I-4E5,分别于注药后5min、15min、30min及1h、2h、6h、24h、48h、72h各处死一组小鼠,取主要脏器称重并测量其放射性计数,计算各脏器每g组织百分注射剂量率(%ID/g).结果:125I标记4E5的标记率为(79.2±2.6)%,放射化学纯度为(97.1±1.1)%,比活度为294.5MBq/mg ;125I-4E5加入到血清及PBS中放置1w后,放化纯度仍>90%;体内分布显示125I-4E5在小鼠体内主要分布于肝、脾、肾,在血液中清除较快.结论:Iodogen法125I标记4E5的标记率和放化纯度高,方法简便,标记物的稳定性好;125I-4E5在小鼠体内主要通过肝和肾代谢,血液中清除较快.
Abstract
Objective To explore the method radiolabeled monoclonal antibody 4E5 with radionuclide 125I, and to observe the biological distribution of 125I-4E5 in normal mice. Methods 4ES was radiolabeled with 125I by the Iodogen method at room temperature . The labeling rate and radiochemical purity was tested with TCA, and the stability of 125I-4E5 was analyzed. 43 mice were divided into nine groups randomly, and 125I-4E5 was injected in the tail vein with 148KBq/0.2 ml separately. The biodistribution of 125I-4E5 was measured at 5min, 15min, 30 min, 1 h, 2 h, 6 h, 24 h, 48 h and 72 h after injection. Results The labeling rate of 125I-4E5 proved(79.2 ±2.6)% , and the radiochemical purity showed(97.1 ±1.1)% . Its specific activity expressed 294.5MBq/mg. The radiochemical purity of 125I-4E5 mixing with serm and PBS indicated over 90% after one week. The body distribution shows 125I-4E5 was deposited mainly in liver, spleen and kidneys, and the blood pool presented 125I-4E5 was eliminated quickly. Conclusion The labeling rate and radiochemical purity of 125I-4E5 using the Iodogen method is high, simple and the stability of 125I-4E5 is fine. The metabolism of 125I-4E5 in normal mice mainly depend on liver and kidneys and with fast clearance in blood.
关键词
碘125/单克隆抗体/标记/生物分布Key words
125I/monoclonal antibody/labeling/biodistribution引用本文复制引用
基金项目
无锡市科技计划项目(CSE01N1221)
出版年
2013
NETL
NSTL
维普
万方数据