摘要
目的:探讨降钙素原(PCT)、纤维蛋白原(Fb)、C反应蛋白(CRP)检测在早期细菌性感染的诊断价值.方法:采用半定量的胶体金免疫结合法检测血清PCT,磁珠凝固法检测血浆Fb,免疫荧光比色法测定全血CRP水平.分别对细菌感染组91例,非细菌感染组108例,非感染组40例(对照组)进行PCT、Fb和CRP的测定.并同时检测白细胞计数和分类.结果:以血清PCT≥0.5ng/ml、Fb>4.0g/L、CRP>8.0mg/L为阳性阈值,细菌感染组PCT的阳性率为98.9%、浓度分别为(0.5～<2.0)、(2.0～<10) ng/ml、≥10 ng/ml 三个级别间;Fb的阳性率为93.4%,浓度为(6.19±1.44)g/L;CRP的阳性率为100%,浓度为(150.5±56.6)mg/L.非细菌感染组PCT的阳性率为18.5%,浓度为(0.5～<2.0)ng/ml;Fb的阳性率为48.1%,浓度为(4.01±1.18)g/L;CRP的阳性率为47.2%,浓度为(48.9±5.61)mg/L.细菌感染组PCT阳性率明显高于非细菌感染组(P<0.01);Fb、CRP水平明显高于非细菌感染组(P<0.01,P<0.01).非细菌感染组Fb、CRP水平明显高于非感染组(2.58±0.32)g/L(P<0.01),CRP(14.5±0.3)mg/L(P<0.01).结论:PCT、Fb、CRP联检可作为早期细菌性感染的敏感诊断指标,指导临床合理用药和治疗.
Abstract
Objective To study the value of procalcitonin(PCT), fibrinogen(Fb) , C-reactive protein (CRP) detection in the early diagnosis of bacterial infection. Methods Semi-quantitative colloidal gold immuno-binding assay for the detection of PCT, magnetic coagulation assay for plasma Fb, immunofluorescence colorimetery of blood CRP level. Bacterial infection group 91 cases, a group of non-bacterial infection 108 cases, 40 cases of non-infection group( control group) measured PCT, Fb and CRP levels respectively. Besides, also detecting white blood cell count and classification simueltaneously. Results serum PCT≥0.5ng/ml, Fb>4.0g/L, CRP > 8.0mg/L as the threshold of positivity, bacterial infection group the positive rate of PCT 98. 9% , concentrationB in 0.5～ <2.0,2.0～<10,≥10 ng/ml three levels respectively the positive rate of Fb 93.4%, concentration af(6.19 ± 1.44) g/L; CRP positive rate 100% , concentration off 150.5 ±56.6) mg/L. Non bacterial infection group the positive rate of PCT 18.5%, at a concentration of 0.5 ～<2.0 ng/ml; the positive rate of Fb 48.1%, concentration of(4.01±1.18) g/L; CRP positive rate was 47.2% , concentration of(48.9±5.61) mg/L. PCT positive rate of bacterial infection group was significantly higher than that of non-bacterial infection group(P<0.01) ; Fb, CKP levels were remarkably higher than the non-bacterial infection group (P<0.01, P <0. 01). Non-bacterial infection group Fb, CKP levels expressed significantly higher than the non-infected group (2.58 ±0.32) g/L, CRP( 14. 50 ±0.31) mg/L(P <0.01). Conclusion PCT, Fb, CRP combined detection can be as early semsitive indicators of diagnosis for bacterial infections and to guide efficient treatment and drug use clinically.
NETL
NSTL
维普
万方数据