血清VEGF及TGF-β1在肝纤维化患者中的表达及其意义
Expression and Significance of Vascular Endothelial Growth Factor and Transforming Growth Factor-β1 in Hepatic Fibrosis
郑盛 1唐映梅 2杨晋辉 2尤丽英2
作者信息
- 1. 云南省第三人民医院消化内科,650011
- 2. 昆明医科大学第二附属医院肝病中心,650000
- 折叠
摘要
目的:探讨血管内皮生长因子(VEGF)及转化生长因子β1(TGF-β1)在肝纤维化患者中的表达及其意义.方法:选择66例肝纤维化患者为观察组,另选择100名体检健康者为正常对照组,采用免疫组织化学方法检测两组血清VEGF及TGF-β1浓度.结果:观察组血清VEGF及TGF-β1浓度为(110.87±22.64)ng/L和(15.08±3.27)μg/L,显著高于对照组(15.98±4.75)ng/L和(7.17±1.86)μg /L(t值分别为20.166、11.066、P均＜0.001);观察组S1、S2、S3、及S4期患者血清VEGF分别为(84.25±16.86)、(101.87±26.70)、(118.04±20.75)、(134.65±25.73)ng/L(F=15.689,P<0.05),TGF-β1分别为(10.87±2.64)、(13.06±2.74)、(17.87±3.28)、(22.76±4.75)μg /L(F=12.438,P<0.05);肝纤维化患者血清VEGF与TGF-β1水平呈正相关(r=0.532,P<0.05).结论:血清VEGF与TGF-β1水平与肝纤维化的发生发展密切相关,二者联检可作为诊断及判断病情的血清学指标.
Abstract
Objective To explore the expression and significance of vascular endothelial growth factor (VEGF) and transforming growth factor-β1 (TGF-β1) in patient with hepatic fibrosis. Methods Sixty-six cases of patients with hepatic fibrosis were selected as observation group and one hundred healthy persons served as control group. Immunohistochemistry were performed to detect VEGF and TGF-β1 in two groups. Results Serum VEGF and TGF-β1 in observation group were significantly higher than those in control group [VEGF:(110.87±22.64) ng/ml vs(15.98 ±4.75) ng/ml, t=20.166, P<0.001; TGF-β1:(15.08 ±3.27) μg/L vs(7.17± 1.86) μg/L, t =11.066, P<0.001]. There were significant differences on VEGF and TGF-β1 level among S1, S2, S3 and S4 subgroups [VEGF:(84.25±16.86) ng/L vs(101.87 ±26.70)ng/L vs(118.04 ±20.75)ng/L vs(134.65 ±25.73)ng/ml, F= 15.689, P<0.05;TGF-β1:(10.87±2.64) μg/L vs(13.06 ±2.74) μg/L vs(17.87 ±3.28) μg/L vs(22.76±4.75) μg/L, F =12.438, P<0.05]. VEGF had positive correlation with TGF-β1(t=0.532, P<0.05). Conclusion Serum VEGF and TGF-β1 levels closely related to the occurrence and development of hepatic fibrosis. Combined detection of serum VEGF and TGF-β1 can be used as serum indexes for diagnosis and evaluation of disease condition.
关键词
肝纤维化/血管内皮生长因子/转化生长因子β1Key words
hepatic fibrosis/vascular endothelial growth factor/transforming growth factor-β1引用本文复制引用
出版年
2013
NETL
NSTL
万方数据