乙型肝炎患者外周血单核细胞CD14+CD16+亚群及HLA-DR的检测及其意义
Significance of Detection of the Monocyte CD14+CD16+ Subsets and HLA-DR in Hepatitis B Patients by FCM
杨喆娟 1董慧慧 2林志益 1李世波 1李绍佐 1刘华生 1李红玲1
作者信息
- 1. 浙江省舟山医院感染性疾病科,316004
- 2. 浙江大学医学院附属第一医院感染科,310003
- 折叠
摘要
目的:通过检测乙型肝炎患者外周血单核细胞各亚群分布情况以及HLA-DR表达水平,探讨慢性乙型肝炎患者外周血中CD14+CD16+单核细胞及HLA-DR的表达特点,以及与疾病进程之间的关系.方法:利用流式细胞仪(FCM) 检测50例慢性乙型肝炎患者,其中21例免疫耐受(IT)患者、29例免疫活化(IA)患者,及31例正常人外周血CD14、CD16、HLA-DR的表达.同时调查肝功能、乙肝五项和血清HBV DNA等相应临床资料.结果:免疫活化组(IA)的CD14+CD16+亚群比率明显高于正常对照组和免疫耐受组(IT)(P<0.01);单核细胞CD14+CD16+亚群HLA-DR表达明显高于CD14++CD16-亚群(P<0.01);乙型肝炎患者CD14+CD16+单核细胞比率与ALT呈正相关(r=0.876,P<0.01),与HBV DNA载量成负相关(r=-0.267,P<0.01).结论:CD14+CD16+单核细胞可能在乙型肝炎患者外周血中参与调节针对HBV的免疫反应,CD14+CD16+单核细胞与HBV复制和肝脏炎症之间存在相关性,因此检测单核细胞CD14+CD16+亚群及HLA-DR水平对于了解乙型肝炎患者疾病进程具有重要的临床应用价值.
Abstract
Objective To investigate the mononuclear cell subsets distribution and HLA-DR of the peripheral blood in hepatitis B patients and analysis the relationship among GD14+ CD16+ monocytes , HLA-DR and the progression of disease. Methods In 50 chronic hepatitis B patients, including 21 patients in immune tolerant phase and 29 patients in immune active phase patients, and 31 normal controls, the expression levels of CD 14 , CD 16, HLA-DR were measured by flow-cytometry. Besides,clinical variables were surveyed for each subjects. Results The expression levels of CD 14 , CD16 on monocyte in group of immune active patients showed significant difference as compared to normal control and patients in immune tolerant phase (P< 0.01) . The expression of HLA-DR on monocyte CD14 + CD16 + subsets proved markedly differences as compared to monocyte CD14 + + CD16-subsets(P <0.01). In chronic hepatitis B patients, elevated proportions of circulating CD14+CD16+ monocytes were correlated with increased serum ALT levels(r = 0.876,P<0.01) and decreased HBV DNA loads(r =-0.267,P<0.01). Conclusion Monocyte CD14 + CD16+ subsets may play an active role in immune response to HBV infection. HBV replication or liver inflammation in chronic HBV infection correlates with the monocyte CD14 + CD16 + subsets. Detection of monocyte CD14 + CD16 + subsets and HLA-DR provides an important clinical value.
关键词
单核细胞CD14+CD16+亚群/HLA-DR/乙型肝炎/流式细胞分析法Key words
monocyte GD14 + CD16+ subsets/HLA-DR/hepatitis B vims/flow cytometry引用本文复制引用
出版年
2013
NETL
NSTL
维普
万方数据