摘要
目的:探讨支气管哮喘患儿治疗前后外周血Th1/Th2相关细胞因子水平的变化及临床意义.方法:应用放射免疫分析和酶联法对31例支气管哮喘患儿进行了外周血中Th1型细胞因子(IL-2、IFN-γ)和Th2型细胞因子IL-4、IL-10浓度,并与35名正常人组作比较.结果:支气管哮喘患儿在治疗前血清IL-2、IFN-γ水平均非常显著地低于正常儿组(P＜0.01),而血清IL-4、IL-10水平又非常显著地高于正常儿组(P＜0.01).经治疗1个月后则与正常儿组比较无显著性差异(P＞0.05).结论:支气管哮喘患儿存在Th1/Th2细胞免疫失衡.Th1免疫功能低下.IFN-γ下调可能是参与哮喘发病机制的重要原因,对哮喘有负向调节作用.哮喘患儿存在Th2细胞免疫功能亢进,以IL-4、IL-10为代表的Th2免疫功能在儿童哮喘的发病中发挥正性促炎作用.
Abstract
Objective To explore the clinical significance on peripheral blood Th1/Th2 related cytokines both before and after treatment in patients with bronchial asthma. Methods Serum IL-2(with RIA), serum IFN-γ,IL-4, IL-10(with ELISA) levels were determined in 31 patients with bronchial asthma and compared to 35 normal controls. Result Before treatment, serum IL-2,IFN-γ, levels showed significantly lower than those in controls(P<0.01) ,but serum IL-4, IL-10 levels were significantly higher(P<0.01) , after treatment for 1 month, the levels expressed significant no difference( (P>0.05). Conclusion Existence of with bronchial asthma imbalance of Th1/Th2 cell immunity in patients with bronchial asthma and furthermore, the hypofunction of Th1 cell immunity, down - regulated IFN-γ may play an important role in the pathogenesis of asthma, it is a negative regulative effect on the development of asthma. Meanwhile, there is a hyperfunction of Th2 cell immunity. IL-4, IL-10 representing. Th2 cell immunity may play a positive promoting inflammatory effect on the development of bronchial asthma in children.
NETL
NSTL
维普
万方数据