T淋巴细胞亚群检测在呼吸系统性疾病的诊断价值
Diagnostic Value of T Lymphocyte Subsets Detection in the Respiratory System Disease
田建良 1王敏2
作者信息
- 1. 绍兴市孙端镇社区卫生服务中心,312000
- 2. 杭州迪安医学检验中心,310000
- 折叠
摘要
目的:探讨T淋巴细胞亚群检测在呼吸系统性疾病的诊断价值.方法:采用流式细胞术对呼吸系统性疾病和正常健康人体内淋巴细胞亚群检测,并通过检测数据分析其淋巴细胞亚群功能变化.结果:①肺结核组、肺炎组和肺癌组的CD3+、CD3+CD4+及T4/T8比值水平均明显低于正常对照组,差异均有极显著变化(P<0.01),而CD3+CD8+水平明显高于正常对照组,差异有极显著变化(P<0.01);②肺结核组、肺炎组与肺癌组T淋巴细胞亚群比较:CD3+、CD3+CD4+及T4/T8比值水平均明显高于肺癌组,差异均有极显著变化(P<0.01),而CD3+CD8+水平明显低于肺癌组,差异有极显著变化(P<0.01);③肺结核组与肺炎组比较:CD3+、CD3+CD4+、CD3+CD8+及T4/T8比值水平无显著性差异(P>0.05).结论:T淋巴细胞亚群参与了呼吸系统性疾病(肺结核、肺炎和肺癌)的病理过程,在其呼吸系统性疾病的发生、发展中起着十分重要的作用.因此,监测呼吸系统性疾病患者外周血T淋巴细胞亚群,可对肺结核和肺炎患者的疗效作出评估和免疫增强提供理论依据,对肺癌患者的辅助免疫治疗支持参照.
Abstract
Objective To explore diagnostic value of T lymphocyte subsets detection in the respiratory system diseases. Methods Flow-cytometry on the respiratory system diseases and normal healthy person's lymphocyte subsets detection, and through the test data to analyse the functional changes of lymphocyte subsets. Results (①pulmonary tuberculosis, pneumonia and lung cancer groups the CD3 + , CD3 + CD4+ and T4/T8 ratio proved significantly lower than those in normal control group, the difference showed markedly changes (P<0.01), and the level of CD3 + CD8 + expressed obviously higher than that of normal control group, the difference indicated significance (P < 0.01) ; ②changes of pulmonary tuberculosis, lung cancer and pneumonia group T lymphocytes comparison of subgroups: CD3+ , CD3 + CD4+ and the ratio of T4/T8 levels showed significantly higher in the lung cancer group, the difference proved significant changes (P<0.01) , and CD3 + CD8+ levels were significantly lower in the lung cancer group, the difference designated obvious changes(P<0.01); ③pulmonary tuberculosis and pneumonia group: CD3+ , GD3+CD4+ , CD3 +CD8 + and T4/T8 ratio indicated no significant difference(P>0.05). Conclusion T lymphocyte subsets participated in the respiratory system disease, tuberculosis, pneumonia and lung cancer) pathological process in the respiratory system disease, plays a important role of development and progress. Therefore, monitoring respiratory system disease patients peripheral blood T lymphocyte subsets detection, might be helpful to tuberculosis and pneumonia patients fo evaluate efficacy and enhancement of immunity provides theoretical basis for lung cancer patients with auxiliary immunotherapy to support the reference.
关键词
T淋巴细胞亚群/流式细胞仪/肺结核/肺炎/肺癌Key words
T lymphocyte subsets/flow cytometry/tuberculosis, pneumonia/lung cancer引用本文复制引用
出版年
2013
NETL
NSTL
维普
万方数据