Simultaneous Determination of Four Aflatoxins in Pheretima by Ultra Performance Liquid Chromatography Tandem Mass Spectrometry and Confirmation of Positive Results
A method for simultaneous determination of aflatoxins B1,B2,G1,and G2 in pheretima was been established by ultra performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS),and the positive results was confirmed.The samples were extracted by 70%methanol,purified by a immunoaffinity columns,separated by UPLC-MS/MS,detected by triple quadrupole mass spectrometry,and confirmed by ion peak ratio.The results showed that the linear relationship of the four aflatoxins were good(r>0.999 5),and the recoveries ranged from 91.2%to 95.6%.The limits of detection for aflatoxin B1,B2,G1,and G2 were 0.10,0.038,0.11,0.038 µg/kg,respectively.The method has a good specificity,high sensitivity,good accuracy,and can be used to determine the final positive detection.Aflatoxin were positively detected in 6 out of 15 batches of pheretima,which proved that the Pheretima were more susceptible to contamination by aflatoxins.
PheretimaUPLC-MS/MSaflatoxinconfirmation of positive results
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