Use,Troubleshooting and Maintenance of Liquid Chromatography Tandem Triple Quadrupole Mass Spectrometry
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维普
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液相色谱串联三重四极杆质谱(liquid chromatography tandem triple quadrupole mass spectrometry,LC-MS/MS)广泛应用于环境分析、食品安全、药物代谢研究、生物医学等领域,主要用于复杂样品基质中痕量目标物检测,既适用于小分子有机化合物定性,又可用于痕量化合物定量.其主要检测模式是多反应监测模式(multiple reaction monitoring,MRM),以定性离子对的丰度比来进行定性,以定量离子对的峰响应进行定量,在MRM检测模式下具有出色的检测灵敏度和定量功能.LC-MS/MS的合理规范使用与维护保养,是保证检测数据准确有效、仪器可持续正常运行的基本要求,也是降低仪器维修成本的重要举措.阐述了安捷伦LC-MS/MS的工作原理、开机与关机、调谐,探讨了常见的故障及应对措施、日常维护保养的内容,旨在为合理规范使用仪器提供参考.
The liquid chromatography tandem triple quadrupole mass spectrometry(LC-MS/MS)is widely used in environmental analysis,food safety,drug metabolism research,biomedicine and other fields.It is mainly used for the detection of trace targets in complex sample matrix,which is suitable for the qualitative of small molecular organic compounds and the quantitative of trace compounds.The main detection mode is multiple reaction monitoring(MRM).It has excellent detection sensitivity and quantitative function in MRM mode by abundance ratio of qualitative ion pair to qualitative and peak response of quantitative ion pair to quantitative.Reasonable standard use and maintenance of the LC-MS/MS is the basic requirement to ensure accurate and effective test data and sustainable normal operation of the instrument,and is also an important measure to reduce the maintenance cost of the instrument.The operating principle,power-on,power-off,tune about the LC-MS/MS of Agilent were described,and the common faults and countermeasures,as well as the contents of daily maintenance were discussed in order to provide a reference for the rational and standardized use of the instrument.
维普
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