首页|用反相高效液相色谱法分离和测定丁烯二酸的顺反异构体

用反相高效液相色谱法分离和测定丁烯二酸的顺反异构体

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  • 国家科技期刊平台
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建立了分离和测定丁烯二酸的2个异构体——顺丁烯二酸和反丁烯二酸的反相高效液相色谱法。以Shim－ pack CLC－ODS 柱为分析柱，以甲醇－水（体积比5∶2，用磷酸调至pH 3.0）为流动相，流速0.7 mL/min, 检测波长为220 nm, 外标法定量。顺、反丁烯二酸的检出限分别为0.10和0.075 mg/L（S/N=2）；相对标准偏差分别为0.47％和0.54％（n=3）。二者的线性范围均为10～60 mg/L。该法具有简便、快速、灵敏度高的特点，用于实际样品测定, 其结果令人满意。
Determination of Two Butendioic Acid Isomers by Reversed phase High performance Liquid Chromatography
A method for the determination of two butendioic acid isomers by reversed phase high performance liquid chromatography is described. cis Butendioic and trans butendioic acids were separated by Shim－pack CLC－ODS column, using methanol－water(5∶2 by volume)solution adjusted to pH 3.0 by phosphoric acid as a mobile phase with flow rate of 0.7 mL/min. The quantitative results are obtained by external standard method. The detection wavelength of 220 nm is applied for the samples. The detection limits are 0.1 mg/L and 0.075 mg/L(S/N=2), and relative standard deviations are 0.47％ and 0.54％(n=3) for cis and trans butendioic acid, respectively. Linear range for both isomers is in 10～60 mg/L. The method is simple, rapid and sensitive, and was applied to determination of practical samples with satisfactory results.

High performance liquid chromatographycis Butendioic acidtrans Butendioic acidIsomer

李金昶、王广、崔秀君、赫奕、孙颜

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东北师范大学分析测试中心，吉林长春 130024

吉林工业大学应用化学系，吉林长春 130025

反相高效液相色谱法 顺丁烯二酸 反丁烯二酸 异构体

2000

分析测试学报
中国广州分析测试中心,中国分析测试协会

分析测试学报

CSTPCDCSCD北大核心
影响因子：1.498
ISSN：1004-4957
年,卷(期)：2000.19(2)
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