Determination of Plant Intrinsic Hormones by Reversed-phase High-performance Liquid Chromatography
A method for the determination of 5 intrinsic hormones:zeatin(ZT), gibberellin(GA3), kinetin(KT), 3-indoleacetic acid(IAA) and abscisic acid(ABA), in plant by reversed-phase high-performance liquid chromatography with μ Bondapak C18 column and UV detector was developed. The optimal chromatographic conditions were selected. An acetonitrile－methanol－0.6 ％ acetic acid system was used as mobile phase and 6-N-benzyl adenine as an internal standard. A method for the extraction of the 5 hormones and the extract preparation was established. A sample of potato was analyzed. The mass fractions of ZT, GA3, KT, IAA and ABA are 0.298 5× 10－9, 253.2× 10－9, 2.154× 10－9, 112.5× 10－9 and 0.652 9× 10－9, respectively. The proposed method is simple, convenient and sensitive.
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维普
