Determination of Five Tetracyclines Residues in Bovine Muscle by UPLC-MS/MS Method
A method for simultaneous analysis of five tetracyclines (tetracycline,oxytetracycline,chlortetracy-cline,doxycycline and minocycline) in bovine muscle was developed by UPLC-MS/MS.The sample was extracted with Mcllvaine buffer-EDTA(pH 4.0) and cleaned up by solid-phase extraction on Oasis HLB cartridges.Analytes were separated by UPLC,and detected by mass spectrum with MRM mode.The calibration curves showed good linear relations in the concentration range of 1-100 μg · L-1 (r＞0.994) for five tetracyclines.The average recoveries at the spiked concentration levels of 1,20,50 and 100 μg·kg-1 were range of 68％-105％and the RSD were less than 34.1％.The LOD of the method was 0.05 μg·kg-1 for five tetracyclines.
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