Analysis of Carotenoids and Their Isomers in Tomatoes by HPLC Method
An analytical method was developed for the determination of carotenoids and their isomers in tomatoes by an improved high performance liquid chromatography (HPLC) method.Hexane-ethanol(3 ∶ 4) was chosen as the best solvent system for extracting carotenoids from tomatoes by stirring 30 min using a magnetic stirring apparatus.11 kinds of carotenoids including all-trans-forms of lycopene,β-carotene,lutein and 8 kinds of cis-isomers were completely separated in 30 min on a Carotenoid C30 column(250 mm ×4.6 mm i.d.,5 μm particle) using methyl tert-butyl ether(MTBE) containing 0.05％ triethylamine (TEA) as mobile phase A and acetonitrile containing 0.05％ TEA as mobile phase B by gradient elution.The flow rate was set at 1.0 mL/min,and the detection wave-length was 475 nm.For lutein,β-carotene and lycopene,the calibration curves were linear in the ranges of 0.1-5.0,0.1-20 and 0.1-100 μg/mL,respectively,with correlation coefficients more than 0.99.The limits of detection (LOD) were 0.13,0.12,0.56 μg/g,respectively,the limits of quantitation (LOQ) were 0.43,0.41,1.88 μg/g,respectively.The intra-day relative standard deviation(RSD) were in the range of 1.2％-5.3％,and the inter-day RSDs were 1.9％-7.5％.The recoveries were in the range of (88.9±6.1)％-(91.7±3.6)％,(92.3±4.3)％-(94.1 ±6.3)％ and (102.6 ±4.2)％-(107.1 ± 3.4)％,respectively.This HPLC method could be used to separate 11 types of carotenoids in tomatoes completely with a short time and high sensitivity.
high performance liquid chromatography(HPLC)carotenoidscis-isomerstomato
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