Synthesis of a Fluorescent Probe and Its Application in Thiols Determination
A bodipy-based fluorescence probe for thiols was developed with high sensitivity and excellent selectivity at a physiological pH (7.4) value.Its structure was characterized by nuclear magnetic resonance(NMR) and high resolution mass spectrometry(HRMS).This probe was designed based on the mechanism that thiols induced the Michael addition reaction to block photo-induced electron transfer process(PET) in this probe.The addition of thiols(0-1 000 μmol/L) to the solution of probe resulted in a remarkable fluorescence enhancement (150-fold) in the green spectra region.Meanwhile,probe was able to detect relatively low concentrations of thiols and the fluorescence detection limit for common amino acids containing thiols,Cys was determined to be 4.5 × 10-7 mol/L.Additionally,similar results were obtained affording 1.2 × 10-7 mol/L and 2.1 × 10-7 mol/L detection limits for GSH and Hcy,respectively.The probe exhibited high selectivity and sensitivity towards thiols over other amino acids.Most importantly,the probe has been successfully utilized for thiols imaging in living cells,which makes it a good candidate for application in biological systems.
国家科技期刊平台
NSTL
万方数据
维普
