Magnetic Solid Phase Extraction Materials for Separation and Enrichment of Phosphoproteins/Peptides and Glycoproteins/Peptides
Protein phosphorylation and glycation are two of the most prevalent post-translational mod-ification processes of proteins.Phosphoproteins and glycoproteins play an important role in the cell life cycle and are also of great significance for the early diagnosis of clinical diseases.However,due to the low content of them and large matrix interference in biological samples,finding an appropriate method for the separation and enrichment of phosphoproteins and glycoproteins is one of the decisive steps in proteomics research.Magnetic solid phase extraction(MSPE)is a solid phase extraction(SPE)technology with magnetic nanoparticles as adsorbent.Compared with conventional SPE,MSPE has the merits of simple operation and environmental friendliness,with its materials easy to re-cycle.Therefore MSPE has been widely applied for the separation and enrichment of phosphoproteins/peptides and glycoproteins/peptides before analysis.In this article,the MSPE materials used for sep-aration and enrichment of phosphoproteins and glycoproteins in recent five years are systematically summarized and reviewed,and the future development trend of such materials is prospected and dis-cussed.
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维普
