Strategies and Applications of Single Cell Proteomics Analysis Based on Mass Spectrometry
Intercellular heterogeneity has been demonstrated to exist in various organisms and their development.Conventionally,the average results obtained from measurements of population cells cannot used to analyze the cellular heterogeneity,so it is important to study the physiological state of single cell at the level of single cells.As proteins are the main carriers of life activities,the analysis of single-cell proteomics is of great significance for the accurate study of the relationship between sig-nal molecules and related signal pathways.Traditional techniques such as immunofluorescence are limited by the binding specificity,availability of antibodies and the analysis of pre-selected proteins.In this paper,the commonly used methods of single-cell proteomics based on mass spectrometry in re-cent years are reviewed.According to different analytical strategies,they are divided into three meth-ods:bottom-up,top-down and native proteomics.Then the development of protein sample pretreat-ment is also discussed.Finally,the challenges and development of single-cell proteomics research based on mass spectrometry are summarized and prospected.
mass spectrometry(MS)proteomicssingle cellsample pretreatment
万方数据
维普
