Analysis of Composition Difference in Breakable Capsules Flavor from Different Batches by SPME-Arrow/GC-MS Combined with Multivariable Statistics
In order to clarify the differences of volatile components in different batch of breakable capsule flavor,a solid phase microextraction arrow and gas chromatography-mass spectrometry(SPME-Arrow/GC-MS)method was established by investigating the type of SPME Arrow,sodium chloride contenet,extraction temperature,equilibrium time and extraction time.The volatile and semi-volatile components in different batch of breakable capsule flavor were analyzed by this method,and the significant differential compounds were analyzed by principal component analysis(PCA),or-thogonal partial least squares-discrimination analysis(OPLS-DA)and F-test.The results showed that,the optimized extraction conditions of SPME arrow were DVB/CAR/PDMS,sodium chloride for 2.0 g,equilibrium for 50 min and extraction for 40 min at 60 ℃.Through spectral library retrieval combined with retention index assisted qualitative analysis,a total of 96 volatile and semi-volatile components were identified.The number of peaks with a relative standard deviation of less than 10％of the target compounds intra-day relative standard deviations(RSDs)accounted for 92.7％of the total peaks.It indicates that the method has good repeatability.A total of 17 potential differential com-pounds were analyzed from different batches of breakable capsule flavor.Through the F-test,10 sig-nificant differential compounds were identified,which were α-pinene,limonene,eucalyptol,isop-ulegol,menthone(including isomers),neoisomenthol,neomenthyl acetate,glycerol tricaprylate and dioctanoic acid monodecanoic acid glyceride.This method can effectively distinguish the internal component differences of different batches of bead samples,and has the characteristics of accuracy,reliability and strong visualization.It can provide technical support for sensory quality inspection of bead products.
万方数据
维普
