Enrichment and Analysis of Trace Microcystins Based on Immobilized Metal Affinity Monolithic Column
The immobilized metal ion affinity chromatography(IMAC)theory was employed to design and synthesize a poly(VIM-co-DVB)-Cu2+monolithic column.The poly(VIM-co-DVB)matrix monolithic column was initially prepared via thermally initiated polymerization of 1-vinylimidazole(VIM)and divinylbenzene(DVB),and Cu2+was immobilized directly on the matrix monolithic col-umn through a post-modification multi-step method.The extraction conditions of the affinity monolith-ic columns were optimized.Under the optimal conditions,the poly(VIM-co-DVB)-Cu2+monolithic column was applied for capillary microextraction(CME)of microcystins(MCs)prior to high perfor-mance liquid chromatography-mass spectrometry(HPLC-MS)analysis in both tap water and lake wa-ter samples.The detection limits of MCs ranged from 0.89 to 1.23 ng/L,with recoveries between 80.0％and 105％,and the relative standard deviations(RSDs)were not more than 6.8％.The vali-dated CME/HPLC-MS methodology exhibits exceptional sensitivity and accuracy,making it a valu-able tool for the monitoring and analysis of MCs in environmental waters.
microcystins(MCs)immobilized metal affinity monolithic columnscapillary micro-extraction(CME)high performance liquid chromatography-mass spectrometry(HPLC-MS)
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