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植物激素对微拟球藻油脂及二十碳五烯酸积累的影响

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考察了6种植物激素对微拟球藻油脂积累与EPA产量的影响,并初步探讨了其对油脂合成的调控机制 .每种植物激素对微拟球藻油脂积累都有促进效果.其中添加20 mg/L IBA 时油脂产量提高最多,油脂质量分数提高20 .94% .其次是添加15 mg/L的BA ,油脂质量分数提高20 .34% .同时,BA在质量浓度为15 mg/L时,EPA质量分数提高了29 .34% .实时定量PCR证实BA等激素能显著提高微拟球藻中油脂合成相关基因的转录水平.证实了植物激素可用于提高微拟球藻EPA的产量,为利用植物激素提高微拟球藻发酵生产EPA提供了实验依据 .
Effects of plant hormones on the lipid and eicosapentaenoic acid production in Nannochloropsis
The effects of six plant hormones on the lipid and EPA production of Nannochloropsis oculata M44 were investigated .Each plant hormone has a beneficial effect on lipid accumulation . When 20 mg/L IBA was added ,the maximum lipid production was achieved with an increase of 20 .94% compared to the control group .The EPA and lipid production were increased by 29 .34%and 20 .34% at a concentration of 15 mg/L BA .Real-time quantitative PCR confirmed that BA and other hormones can significantly increase the transcriptional level of lipid synthesis-related genes in Nannochloropsis oculata M44 .This study confirms that plant hormones can be used to increase the EPA production of Nannochloropsis oculata M44 ,which provides the experimental basis for the production of EPA .

eicosapentaenoic acidplant hormoneslipid synthesisNannochloropsisreal-time quantitative PCR

李悦明、崔球、徐建春、宋晓金、夏修峦、李霞、范栋、李妍

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青岛琅琊台集团股份有限公司,山东 青岛 266400

中国科学院青岛生物能源与过程研究所,山东 青岛 266101

黄岛出入境检验检疫局,山东 青岛 266400

青岛市黄岛区食品药品监督管理局,山东 青岛 266400

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二十碳五烯酸 植物激素 油脂合成 微拟球藻 实时定量PCR

工信部绿色制造系统集成项目青岛市自主创新重大专项

Z13506000900216-7-1-11-zdzx-nsh

2018

发酵科技通讯
中国发酵工业协会,杭州西湖味精集团有限公司

发酵科技通讯

影响因子:0.311
ISSN:1674-2214
年,卷(期):2018.47(2)
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