A simple and accurate detection method for multiple illegal laxative additives in cooling fruit enzyme products has been established using UPLC-MS/MS.Sample pulverization was achieved through ultra-micro cryogenic technology,followed by purification using Oasis® PRIME HLB solid-phase extraction(SPE).The UPLC-MS/MS method employed a gradient elution with a mobile phase consisting of a 0.02 mol/L ammonium acetate aqueous solution and methanol.Separation was carried out using a CORTECS® UPLC C18(1.6 μm,2.1 mm×100 mm)chromatographic column.Quantitative detection of six illegal laxative additives in cooling fruit enzyme products was conducted using a tandem quadrupole mass spectrometer.Results:the linear range was 1～1 000 μg/L,correlation r2≥0.996 1;the detection limits of LOD were 0.1～1.0μg/kg,the limits of quantification of LOQ were 0.3～3.0 μg/kg,and the average recoveries were 82.5%～105.3%.Intraday precision RSD(n=9)ranged from 0.8%to 3.7%.The method has strong specificity and fast operation,and can be used as an effective method for the detection of compounds causing illegal laxative additives in cold fruit enzymes.
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