Two strains of Cordyceps endophytic fungi were fermented and cultured to study the extraction process of superoxide dismutase(SOD)from the Cordyceps endophytic fungi.The SOD extraction technology of 2 strains of Cordyceps endophytic fungi(No.01 and No.02)isolated from Cordyceps sinensis was studied by orthogonal experiment with enzyme activity as index.The experimental results showed the best extraction process of SOD of strain No.01 was as follows:ultrasonic extraction time of 45 min,liquid-solid ratio of 40 mL/g,pH of 7.0,and the activity of 421 U.The optimal extraction process of strain No.02 was as follows:ultrasonic extraction time of 75 min,liquid-solid ratio of 30 mL/g,pH of 8.0,and the activity of 362 U.By comparing No.01 and No.02,it can be concluded that Cordyceps endophytes contain abundant SOD.Due to the two different origins,the SOD vitality of Cordyceps endophytic fungi varied greatly,and the extraction process was also substantial different.
Cordyceps endophytic fungienzyme activitySODextraction process
维普
万方数据
