发酵科技通讯2024,Vol.53Issue(3) :125-130.

大曲中酵母菌组成及优势酵母的功能特性

Composition of Saccharomyces cerevisiae and functional properties of dominant yeasts in Daqu

石馨 侯小歌 韩明杰 张方利 惠明
发酵科技通讯2024,Vol.53Issue(3) :125-130.

大曲中酵母菌组成及优势酵母的功能特性

Composition of Saccharomyces cerevisiae and functional properties of dominant yeasts in Daqu

石馨 1侯小歌 2韩明杰 2张方利 3惠明1
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作者信息

  • 1. 河南工业大学 生物工程学院,河南 郑州 450001
  • 2. 河南牧业经济学院 食品与生物工程学院,河南 郑州 450046
  • 3. 贵州黔酒股份有限公司,贵州 仁怀 564507
  • 折叠

摘要

酵母菌是酿酒大曲的主要功能菌之一,研究不同种类大曲的酵母菌群及优势酵母的发酵特性,有助于制曲工艺控制及酿酒功能菌的筛选与应用.以成熟高温大曲、中高温大曲和低温大曲为试材,对大曲中的酵母菌进行分离纯化和分子鉴定,初步探索优势酵母菌的耐温、耐酸、耐酒精及产酶特性.从三类大曲中各获得1株优势酵母菌,经鉴定分别为酿酒酵母(编号:GY1)和异常威克汉姆酵母种(编号:ZY1,DY1).3株优势酵母菌均对酒精有一定的耐受能力,均具备产生果胶酶、淀粉酶、纤维素酶、蛋白酶及酯化酶的能力,可为酿酒产香功能酵母菌的筛选及生产应用提供菌株资源.

Abstract

Saccharomyces cerevisiae is one of the main functional bacteria in Daqu .Studying the yeast groups of different species of Daqu and the fermentation characteristics of the dominant yeast is helpful to the control of Daqu production process and the screening and application of functional bacteria of Daqu . Using mature high-temperature Daqu,medium-temperature Daqu and low-temperature Daqu as test materials,the yeast in Daqu was isolated,purified and molecular identified,and the characteristics of temperature,acid,alcohol and enzyme production of the dominant yeast were preliminarily explored .One dominant yeast strain was obtained from each of the three types of Daqu . Saccharomyces cerevisiae (Number:GY1) and Wickerhamomyces (Number:ZY1,DY1) were identified .All the three strains of dominant yeast had a certain tolerance to alcohol,and the ability to produce pectinase,amylase,cellulase,protease and esterification enzyme,w hich could provide strain resources for the screening and production of flavor-producing yeast in liquor .

关键词

大曲/酵母菌/菌株/培养基/透明圈

Key words

Daqu/Saccharomyces cerevisiae/bacterial strain/substrate/transparent zone

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基金项目

河南省重大科技专项课题(181100211400-8-2)

出版年

2024
发酵科技通讯
中国发酵工业协会,杭州西湖味精集团有限公司

发酵科技通讯

影响因子:0.311
ISSN:1674-2214
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