As a freshwater alga,Ochromonas malhamensis can be used to produce water-soluble β-1,3-glucan .In order to increase the biomass and yield of water-soluble β-1,3-glucan of Ochromonas malhamensis,the heterotrophic medium and conditions of Ochromonas malhamensis were optimized in 7 .5 L fermenter,and the process was scaled up in 125 L fermenter . The experimental results showed that with optimized medium and culture conditions,the biomass of Ochromonas malhamensis cultured in 7 .5 L fermenter reached 61 .25 g/L,which was 2 .4 times higher than that before optimization . In 125 L fermenter,the biomass of Ochromonas malhamensis was not significantly different from that of 7.5 L fermenter .The average production rate of water-soluble β-1,3-glucan reached 0.36 g/(L·h),which was 1.8 times higher than that reported in the literature .This study provided a basis for the production of β-1,3-glucan by high-cell-density heterotrophic cultivation of Ochromonas malhamensis,and laid a foundation for its application in food,medicine and feed .
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