摘要
蔬菜中的农药残留对人体的危害一直是老百姓十分关注的问题,职能部门非常重视快检在蔬菜中的应用,然而由于国家标准采用的酶抑制率法对部分农药检测容易受干扰,因此开发免疫金快检方法十分重要.以生姜为研究对象,开展了不同预处理方式对生姜中的克百威、灭多威、丙溴磷、甲基异柳磷和辛硫磷快检方法及分析.实验结果表明:相较于酶抑制率法,生姜预处理会对有机磷和氨基甲酸酯类检测试剂产生干扰,尤其是生姜在匀浆、剪碎状态下会产生干扰,且影响最大;在匀浆、剪碎和整株状态下,对生姜中的克百威、灭多威、丙溴磷、甲基异柳磷和辛硫磷采用胶体金5联快速检测无干扰,说明胶体金方法较酶抑制率法对生姜更具备实用性.
Abstract
The harm of pesticide residues in vegetables to the human body has always been a matter of great concern to the people,and functional departments attach great importance to the application of rapid detection methods in vegetables .However,the enzyme inhibition rate method adopted by the national standard is easy to interfere with the detection of some pesticides . Therefore,it is crucial to develop the immunogold rapid detection method .Using ginger as the research object,different pre-treatment methods were carried out for the quick detection and analysis of ginger carbofuran,methomyl,probromophos,methylisophoxim,and phoxim .The experimental results showed that ginger pre-treatment would interfere with organic phosphorus and carbamate detection reagents,especially when ginger was in a homogenized or shredded state,w hen compared to the enzyme inhibition rate method . By using colloidal gold rapid detection,ginger has no interference and strong anti-interference ability to the five linked rapid detection cards of carbofuran,methomyl,probromophos,methylisophoxim,and phoxim in ginger under homogenization,shredding,and w hole plant conditions,indicating that the colloidal gold method was more practical than the enzyme inhibition rate method for ginger preparation .
基金项目
杭州市农业和社会发展重点基金资助项目(20230701A04)
国家市场监督管理总局科技计划项目(2023MK165)
浙江省重点研发项目(2023C02042)
浙江省市场管理局雏鹰培育处级计划项目(CY2022345)
维普
万方数据