The filter paper method was used to screen strains with antimicrobial activity,and the active strains were identified,the optimization of fermentation conditions was also conducted . The results showed that three strains of Streptomyces with different antibacterial activities were obtained,among which the fermentation broth of strain ZJPH2021031 showed higher inhibitory activity against Staphylococcus aureus than that of streptomycin sulfate at a concentration of 50 mmol/L .The fermentation broth of strain ZJPH2021032 inhibits the growth of Gram-positive bacteria and fungi,while the fermentation broth of ZJPH2021033 only inhibited the growth of fungi .Meanwhile,the inhibitory activity of the fermentation broth of strain ZJPH2021031 against Staphylococcus aureus was significantly enhanced,and the bacteriostatic diameter increased by 2 .16-fold w hen fermented in a medium containing various metal ions and amino acids only as a nitrogen source . Additionally,the glycerol content of the medium was the most important for the production of antimicrobial active substances .
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